ABSTRACT

Blood is the most interesting tissue for molecular analysis in a wide range of diseases since it provides accumulative information of the whole body state. Transcriptomic analysis of the blood is one of the most favorable omics approaches for clinical and research purposes. However, blood sample preparation for RNA analyses is highly diverse encompassing specific methodology and application. In this study, we review the common sample preparation maneuvers including RNA handling techniques, sample collection and stabilization, blood cell sorting, extracellular vesicle enrichment, RNA extraction, globin/ribosomal RNA reduction, and RNA concentration and quality assessment. These techniques are the most prevalent procedures of blood sample preparation in RNA transcriptomic analyses. The methodologies, technical challenges of the current approaches, and latest advances in the field are discussed in detail.

摘要

血液是对多种疾病进行分子分析最有趣的组织，因为它提供了全身状态的累积信息。血液的转录组学分析是用于临床和研究目的的最有利的组学方法之一。然而，用于 RNA 分析的血液样本制备非常多样化，包括特定的方法和应用。在本研究中，我们回顾了常见的样品制备操作，包括 RNA 处理技术、样品收集和稳定、血细胞分选、细胞外囊泡富集、RNA 提取、珠蛋白/核糖体 RNA 减少以及 RNA 浓度和质量评估。这些技术是 RNA 转录组分析中最普遍的血样制备程序。当前方法的方法论、技术挑战，