Lactate is an important metabolite generated during the culture of pluripotent stem cells and is known to inhibit the cell growth. With the increase in the prominence of pluripotent stem cell applications, reliable methods are required to remove metabolites such as lactate for enabling the high-density culture of cells. Herein, we report an investigation of layered double hydroxides (LDHs) and layered double oxides (LDOs) as anion-adsorbents for lactate removal to regenerate the cell culture medium. The lactate absorption ability of Cu-Al and Mg-Al LDH and Cu-Al and Mg-Al LDO adsorbents was evaluated. The influence of various process parameters, including temperature and pH, on the lactate adsorption, were investigated. The kinetics of the adsorption process and equilibrium dynamics were studied, and the maximum adsorption amounts were determined. The Cu-Al LDH, Cu-Al LDO, Mg-Al LDH, and Mg-Al LDO exhibited a lactate adsorption that followed a pseudo-second-order kinetic model, and their apparent activation energies for adsorption were 32.0, 47.4, 95.7, and 61.7 kJ/mol, respectively; these values indicated that chemical adsorption was the underlying mechanism of adsorption. The Langmuir and Freundlich models were analyzed and revealed the nature of the anion-exchange process. This study is expected to promote the high-density culture of cells by ensuring the regeneration of the media by the efficient removal of the undesired lactate.

乳酸是在多能干细胞培养过程中产生的重要代谢产物，已知抑制细胞生长。随着多能干细胞应用重要性的增加，需要可靠的方法来去除代谢产物，例如乳酸，以实现细胞的高密度培养。在本文中，我们报告了对层状双氢氧化物（LDHs）和层状双氧化物（LDOs）作为阴离子吸附剂进行乳酸去除以再生细胞培养基的研究。评价了Cu-Al和Mg-Al LDH以及Cu-Al和Mg-Al LDO吸附剂的乳酸吸收能力。研究了包括温度和pH在内的各种工艺参数对乳酸吸附的影响。研究了吸附过程的动力学和平衡动力学，并确定了最大吸附量。Cu-Al LDH，Cu-Al LDO，Mg-Al LDH和Mg-Al LDO表现出遵循伪二级动力学模型的乳酸吸附，其表观活化能为32.0、47.4、95.7，和分别为61.7 kJ / mol; 这些值表明化学吸附是吸附的基本机理。分析了Langmuir和Freundlich模型，并揭示了阴离子交换过程的性质。期望通过有效去除不需要的乳酸来确保培养基的再生，从而促进细胞的高密度培养。这些值表明化学吸附是吸附的基本机理。分析了Langmuir和Freundlich模型，并揭示了阴离子交换过程的性质。期望通过有效去除不需要的乳酸来确保培养基的再生，从而促进细胞的高密度培养。这些值表明化学吸附是吸附的基本机理。分析了Langmuir和Freundlich模型，并揭示了阴离子交换过程的性质。期望通过有效去除不需要的乳酸来确保培养基的再生，从而促进细胞的高密度培养。