The use of synthetic pesticide carries along several disadvantages talking about the preservation of the natural homeostasis of the planet, causing the searching of biopesticide, which one presents advantages as well as biodegradability in minimum possible time, the low toxicity in comparison to synthetic pesticides and their variety of structure, which allows slowing down the appearance of resistance. The aim of this work was to evaluate the effect on the larvicidal action when artificially varying the chemical composition of orange oil (Citrus sinensis L.). As results, we found that the analysis of gas chromatography coupled to mass spectrometry showed the presence of terpenoid and sesquiterpenoid compounds in the different samples. The use of electric pulses on samples modified their chemical composition, so that the percentage of limonene went from 72% in the sample that was not subjected to electric treatment to lower percentages, even in sample three the percentage of limonene was <50%. Only three compounds (limonene, linalool and caryophyllene) were found to be common in all samples. Subsequently, the larvicidal action on Drosophila melanogaster larvae was evaluated. Six concentrations of each oil sample were tested (0, 100, 500, 1000, 5000 and 10000 ppm). We found that there was no linear relationship between concentration and lethality. Additionally, in the sample without electrical treatment most of the concentrations tested had lethality higher than 50%, while in sample 7 the results of the lethality were lower than 30%, so that biological tests showed that in samples where the concentration of limonene was lower, the lethality in the larvae decreased.

合成农药的使用带来了几个缺点，这些缺点涉及到行星的自然稳态的保存，从而导致了对生物农药的搜索，这显示了其优点以及在尽可能短的时间内可生物降解，与合成农药相比毒性低以及它们的优点。多种结构，可以减慢电阻的出现。这项工作的目的是评估人为改变橙油（柑桔）化学成分时对杀幼虫作用的影响。L.）。结果，我们发现，气相色谱与质谱联用分析表明，不同样品中存在萜类和倍半萜类化合物。在样品上使用电脉冲会改变其化学成分，从而使柠檬烯的比例从未经电处理的样品中的72％降至更低的百分比，即使在样品3中，柠檬烯的比例也小于50％。在所有样品中仅发现三种化合物（柠檬烯，芳樟醇和石竹烯）是常见的。随后，对果蝇果蝇的杀幼虫作用评估幼虫。测试了每种油样品的六个浓度（0、100、500、1000、5000和10000 ppm）。我们发现浓度与致死率之间没有线性关系。此外，在未经电处理的样品中，大多数测试浓度的致死率均高于50％，而在样品7中，致死率的结果均低于30％，因此生物学测试表明，在柠檬烯浓度较低的样品中，其致死率低于30％。 ，幼虫的致死率降低。