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Bacillus subtilis KLBC BS6 induces resistance and defence-related response against Botrytis cinerea in blueberry fruit
Physiological and Molecular Plant Pathology ( IF 2.8 ) Pub Date : 2020-12-31 , DOI: 10.1016/j.pmpp.2020.101599
Yanyuan Lu , Diantong Ma , Xin He , Fang Wang , Jianrong Wu , Yun Liu , Junying Jiao , Jia Deng

The potential biocontrol strain Bacillus subtilis KLBC BS6 was isolated from blueberry rhizosphere soil, and its efficacy for controlling grey mould caused by Botrytis cinerea in postharvest blueberry fruit and the underlying possible disease resistance mechanisms were investigated. Treatment with B. subtilis KLBC BS6 was expected to inhibit the mycelial growth and conidial germination of B.cinerea in vitro. The incidence of grey mould in blueberry fruit treated with B. subtilis KLBC BS6 (and inoculated with B. cinerea) was decreased compared with that in the control fruit after 4 days of incubation, regardless of the preventive or curative nature of the treatment. Moreover, preventive treatment was more effective than the curative one in controlling of grey mould-induced decay. In B. subtilis KLBC BS6-treated blueberry fruit, the activities of disease resistance-related enzymes (chitinase, phenylalanine ammonia lyase, peroxidase, and polyphenol oxidase) were significantly enhanced. Furthermore, compared to the control fruit, the B. subtilis KLBC BS6-treated blueberry fruit exhibited unimpaired internal fruit quality and maintained high titratable acidity and ascorbic acid content. Thus, B. subtilis KLBC BS6 is a suitable biocontrol agent for prolonging the postharvest shelf-life of blueberry fruit and other horticultural products.



中文翻译:

枯草芽孢杆菌KLBC BS6诱导蓝莓果实中灰葡萄孢的抗性和防御相关反应

从蓝莓根际土壤中分离到了潜在的枯草芽孢杆菌KLBC BS6,研究了其对收获后蓝莓果实中灰葡萄孢引起的灰霉病的防治效果及其潜在的抗病机理。治疗枯草芽孢杆菌KLBC BS6预计将抑制菌丝生长和分生孢子萌发的体外灰霉病菌。用枯草芽孢杆菌KLBC BS6(和灰葡萄孢接种)处理的蓝莓果实中灰霉病的发生率孵育4天后,与对照果实相比,)降低了,无论该处理是预防还是治愈。此外,在控制灰霉病引起的衰老中,预防性治疗比治疗性治疗更有效。在枯草芽孢杆菌KLBC BS6处理的蓝莓果实中,与疾病抗性相关的酶(几丁质酶,苯丙氨酸氨裂合酶,过氧化物酶和多酚氧化酶)的活性显着增强。此外,与对照果实相比,枯草芽孢杆菌KLBC BS6处理的蓝莓果实显示出良好的内部果实品质,并保持了高滴定酸度和抗坏血酸含量。因此,枯草芽孢杆菌 KLBC BS6是延长蓝莓果实和其他园艺产品收获后货架期的合适生物防治剂。

更新日期:2021-02-09
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