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Transport stress in bullfrog: Hematological and plasma biochemical responses
Aquaculture Reports ( IF 3.2 ) Pub Date : 2020-12-31 , DOI: 10.1016/j.aqrep.2020.100583
Bruno Dias dos Santos , Adriana Xavier Alves , Nayara Netto dos Santos , Mariele Lana , Victor Ramos Pawlowski , Renan Rosa Paulino , Fabíola de Oliveira Paes Leme , Frederico Augusto de Alcântara Costa , Marcos Ferreira Brabo , Daniel Abreu Vasconcelos Campelo , Galileu Crovatto Veras

This work aims to evaluate the effect of transport stress on the hematological and biochemical responses in males and females of bullfrogs (Lithobates catesbeianus). Frog conditions were evaluated before and at five times after transport (0, 6, 12, 24, and 48 h) for both genders. Glycemia increased in the frogs evaluated at time 0 h and recovered 24 h after transport. Lactate increased in the animals evaluated at time 0 h, recovered at time 6 h and reduced 48 h after transport. Aspartate-aminotransferase (AST) increased in animals analysed 0, 6, and 12 h and recovered 24 h after transport. Alanine-aminotransferase (ALT) increased in the frogs analysed at time 0 h, recovered at time 6 h and decrease 48 h after transport. Total proteins, albumin and globulins decreased 48 h after transport. Triglycerides and total cholesterol decrease in the animals evaluated 24 and 48 h after transport. Males had high levels of total cholesterol. Number of erythrocytes increased in the animals evaluated 0 and 6 h after transport and recovered 12 h after transport. Mean corpuscular volume (MCV) of the frogs evaluated 0 and 6 h after transport were lower and recovered 12 h after transport. Hematocrit and hemoglobin decrease 48 h after transport. Mean corpuscular hemoglobin (MCH) decreased in the animals evaluated 0, 6, and 12 h and recovered 24 h after transport. Frogs demonstrated rapid recovery of homeostasis after transport, between 6 and 24 h. Between 24 and 48 h after transport, a decrease in energy reserves and red blood series is observed, caused by prolonged fasting.



中文翻译:

牛蛙的运输应激:血液学和血浆生化反应

这项工作旨在评估运输压力对牛蛙雌雄(Lithobates catesbeianus)血液学和生化反应的影响)。在两个性别的运输之前和之后五次(0、6、12、24和48小时)评估青蛙的状况。在0小时的时间评估青蛙体内的血糖升高,并在运输24小时后恢复。在0小时评估的动物中,乳酸增加,在6小时恢复,在运输后48小时减少。在0、6和12小时分析的动物中,天冬氨酸转氨酶(AST)增加,运输后24小时恢复。青蛙的丙氨酸氨基转移酶(ALT)在0小时时增加,在6小时时恢复,在运输后48小时减少。运输后48小时,总蛋白,白蛋白和球蛋白下降。运输后24和48小时评估动物的甘油三酸酯和总胆固醇降低。男性的总胆固醇水平很高。运输后0和6小时评估的动物中红细胞数量增加,运输后12小时恢复。运输后0小时和6小时评估的青蛙的平均红细胞体积(MCV)较低,运输后12小时恢复。运输后48小时血细胞比容和血红蛋白减少。在评估的0、6和12 h的动物中平均血红蛋白(MCH)下降,并在运输后24 h恢复。青蛙在运输后6至24小时内表现出体内稳态的快速恢复。运输后24至48小时之间,由于禁食时间延长,能量储备和红血系列减少。运输后48小时血细胞比容和血红蛋白减少。在评估的0、6和12 h的动物中平均血红蛋白(MCH)下降,并在运输后24 h恢复。青蛙在运输后6至24小时内表现出体内稳态的快速恢复。运输后24至48小时之间,由于禁食时间延长,能量储备和红血系列减少。运输后48小时血细胞比容和血红蛋白减少。在评估的0、6和12 h的动物中平均血红蛋白(MCH)下降,并在运输后24 h恢复。青蛙在运输后6至24小时内表现出体内稳态的快速恢复。运输后24至48小时之间,由于禁食时间延长,能量储备和红血系列减少。

更新日期:2020-12-31
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