Mesenchymal stem cells (MSCs) have immense therapeutic potential for treating intractable and immune diseases. They also have applications in regenerative medicine in which distinct treatments do not exist. Thus, MSCs are gaining attention as important raw materials in the field of cell therapy. Importantly, the number of MSCs in the bone marrow is limited and they are present only in small quantities. Therefore, mass production of MSCs through long-term culture is necessary to use them in cell therapy. However, MSCs undergo cellular senescence through repeated passages during mass production. In this study, we explored methods to prolong the limited lifetime of MSCs by culturing them with different seeding densities.

中文翻译：

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低细胞密度培养可延缓长期培养中人骨髓来源的间充质干细胞的细胞衰老。

间充质干细胞 (MSCs) 在治疗顽固性疾病和免疫疾病方面具有巨大的治疗潜力。它们还应用于不存在不同治疗方法的再生医学中。因此，间充质干细胞作为细胞治疗领域的重要原料而受到关注。重要的是，骨髓中 MSC 的数量是有限的，并且它们仅以少量存在。因此，需要通过长期培养大量生产 MSCs 才能将其用于细胞治疗。然而，MSCs 在大规模生产过程中通过反复传代经历细胞衰老。在这项研究中，我们探索了通过培养不同接种密度来延长 MSCs 有限寿命的方法。