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Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples
Journal of Analytical Methods in Chemistry ( IF 2.3 ) Pub Date : 2020-12-30 , DOI: 10.1155/2020/8810726
Yong-Hyun Kim 1, 2 , Jeongsik Park 1
Affiliation  

Human beings are easily exposed to formaldehyde (FA) in a living environment. Entry of FA into the human body can have adverse effects on human health, depending on the FA concentration. Thus, a quantitative analysis of FA in blood is necessary in order to estimate its effect on the human body. In this study, a simple and rapid analytical method for the quantitation of FA in blood was developed. The total analysis time, including the pretreatment procedure, was less than 20 min. To ensure a stable analysis, blood samples were stabilized using tripotassium ethylenediaminetetraacetic acid solution, and FA was selectively derivatized using 2,4-dinitrophenylhydrazine as pretreatment procedures. The pretreated samples were analyzed using a high-performance liquid chromatography-UV system, which is the most common choice for analyzing small-molecule aldehydes like formaldehyde. Verification of the pretreatment methods (stabilization and derivatization) using FA standards confirmed that the pretreatment methods are highly reliable in the calibration range 0.012–5.761 ng μL–1 (slope = 684,898, R2 = 0.9998, and limit of detection = 0.251 pg·μL–1). Analysis of FA in the blood samples of a Yucatan minipig using the new method revealed an average FA concentration of 1.98 ± 0.34 ng μL–1 (n = 3). Blood samples spiked with FA standards were analyzed, and the FA concentrations were found to be similar to the theoretical concentrations (2.16 ± 0.81% difference). The method reported herein can quantitatively analyze FA in blood at a sub-nanogram level within a short period of time and is validated for application in blood analysis.

中文翻译:


开发一种简单而强大的血液样本中甲醛检测和定量分析方法



人类在生活环境中很容易接触到甲醛(FA)。 FA 进入人体会对人体健康产生不利影响,具体取决于 FA 浓度。因此,有必要对血液中的 FA 进行定量分析,以评估其对人体的影响。在本研究中,开发了一种简单快速的血液中 FA 定量分析方法。总分析时间(包括预处理过程)不到 20 分钟。为了确保分析稳定,使用乙二胺四乙酸三钾溶液稳定血样,并使用 2,4-二硝基苯肼作为预处理程序选择性衍生 FA。使用高效液相色谱-紫外系统对预处理的样品进行分析,这是分析甲醛等小分子醛类的最常见选择。使用 FA 标准对预处理方法(稳定化和衍生化)进行验证,证实预处理方法在校准范围 0.012–5.761 ng μ L –1内高度可靠(斜率 = 684,898, R 2 = 0.9998,检测限 = 0.251 pg) · μ L –1 )。使用新方法对尤卡坦小型猪血液样本中的 FA 进行分析,结果显示平均 FA 浓度为 1.98 ± 0.34 ng μL –1 ( n = 3)。对添加 FA 标准品的血样进行分析,发现 FA 浓度与理论浓度相似(差异为 2.16 ± 0.81%)。本文报道的方法可以在短时间内以亚纳克水平定量分析血液中的FA,并在血液分析中得到验证。
更新日期:2020-12-30
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