Human beings are easily exposed to formaldehyde (FA) in a living environment. Entry of FA into the human body can have adverse effects on human health, depending on the FA concentration. Thus, a quantitative analysis of FA in blood is necessary in order to estimate its effect on the human body. In this study, a simple and rapid analytical method for the quantitation of FA in blood was developed. The total analysis time, including the pretreatment procedure, was less than 20 min. To ensure a stable analysis, blood samples were stabilized using tripotassium ethylenediaminetetraacetic acid solution, and FA was selectively derivatized using 2,4-dinitrophenylhydrazine as pretreatment procedures. The pretreated samples were analyzed using a high-performance liquid chromatography-UV system, which is the most common choice for analyzing small-molecule aldehydes like formaldehyde. Verification of the pretreatment methods (stabilization and derivatization) using FA standards confirmed that the pretreatment methods are highly reliable in the calibration range 0.012–5.761 ng μL^–1 (slope = 684,898, R² = 0.9998, and limit of detection = 0.251 pg·μL^–1). Analysis of FA in the blood samples of a Yucatan minipig using the new method revealed an average FA concentration of 1.98 ± 0.34 ng μL^–1 (n = 3). Blood samples spiked with FA standards were analyzed, and the FA concentrations were found to be similar to the theoretical concentrations (2.16 ± 0.81% difference). The method reported herein can quantitatively analyze FA in blood at a sub-nanogram level within a short period of time and is validated for application in blood analysis.

中文翻译：

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开发一种简单而强大的血液样本中甲醛检测和定量分析方法


人类在生活环境中很容易接触到甲醛（FA）。 FA 进入人体会对人体健康产生不利影响，具体取决于 FA 浓度。因此，有必要对血液中的 FA 进行定量分析，以评估其对人体的影响。在本研究中，开发了一种简单快速的血液中 FA 定量分析方法。总分析时间（包括预处理过程）不到 20 分钟。为了确保分析稳定，使用乙二胺四乙酸三钾溶液稳定血样，并使用 2,4-二硝基苯肼作为预处理程序选择性衍生 FA。使用高效液相色谱-紫外系统对预处理的样品进行分析，这是分析甲醛等小分子醛类的最常见选择。使用 FA 标准对预处理方法（稳定化和衍生化）进行验证，证实预处理方法在校准范围 0.012–5.761 ng μ L ^–1内高度可靠（斜率 = 684,898， R ² = 0.9998，检测限 = 0.251 pg） · μ L ^–1 )。使用新方法对尤卡坦小型猪血液样本中的 FA 进行分析，结果显示平均 FA 浓度为 1.98 ± 0.34 ng μL ^–1 ( n = 3)。对添加 FA 标准品的血样进行分析，发现 FA 浓度与理论浓度相似（差异为 2.16 ± 0.81%）。本文报道的方法可以在短时间内以亚纳克水平定量分析血液中的FA，并在血液分析中得到验证。