3D Imaging and Quantification of the Integrin at a Single-Cell Base on a Multisignal Nanoprobe and Synchrotron Radiation Soft X-ray Tomography Microscopy,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

3D Imaging and Quantification of the Integrin at a Single-Cell Base on a Multisignal Nanoprobe and Synchrotron Radiation Soft X-ray Tomography Microscopy
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-12-30 , DOI: 10.1021/acs.analchem.0c04662
Chunyu Zhang _{1,

2} , Shengkun Yao _{3,

4} , Chao Xu ₅ , Yanan Chang ₆ , Yunbing Zong ₃ , Kai Zhang ₇ , Xiangzhi Zhang ₈ , Lijuan Zhang ₈ , Chunying Chen ₁ , Yuliang Zhao ₁ , Huaidong Jiang ₃ , Xueyun Gao ₂ , Yaling Wang ₁

Affiliation

CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing 100090, China
Department of Chemistry and Chemical Engineering, Beijing University of Technology, Beijing 100124, China
School of Physical Science and Technology, Shanghai Tech University, Shanghai 201210, China
Shandong Provincial Engineering and Technical Center of Light Manipulations & Shandong Provincial Key Laboratory of Optics and Photonic Device, School of Physics and Electronics, Shandong Normal University, Jinan 250358, China
College of Chemistry and Material Science, Shandong Agricultural University, Tai’an 271018, China
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

The development of three-dimensional (3D) single-cell imaging and protein quantitative methods can provide more comprehensive information for diagnoses. We report the design and synthesis of a multisignal nanoprobe (AuGd_NC@BSA-CV) for single-cell 3D imaging and quantifying the integrin α_IIbβ₃ using correlated synchrotron radiation soft X-ray tomography microscopy and an iterative tomographic algorithm termed equally sloped tomography for the first time. Moreover, on the basis of the Au or Gd content of our nanoprobe, the number of integrin α_IIbβ₃ on a single cell also can be accurately quantified (1.5 × 10⁷ per cell) via inductively coupled plasma mass spectrometry.

中文翻译：

3D成像和整合素在多信号纳米探针和同步辐射软X射线断层扫描显微镜的单细胞基础上的量化。

三维（3D）单细胞成像和蛋白质定量方法的发展可以为诊断提供更全面的信息。我们报告一个多信号纳米探针的设计和合成（AuGd _NC @ BSA-CV）为单细胞的3D成像和定量整合素α _IIB β ₃使用相关同步辐射软X射线断层摄影显微镜和迭代断层算法地称为同样倾斜首次进行断层扫描。此外，我们的纳米探针的金或Gd含量的基础上，整合素α的数量_IIB β ₃在单个细胞上也可以精确地定量（1.5×10 ⁷每细胞），通过电感耦合等离子体质谱法。

更新日期：2021-01-26

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11