Introduction: Bluetongue disease is an economically important viral disease of livestock caused by bluetongue virus (BTV) having multiple serotypes. It belongs to the genus Orbivirus of family Reoviridae and subfamily Sedoreovirinae. The genome of BTV is 10 segmented dsRNA that codes for 7 structural and 4 nonstructural proteins, of which VP2 was reported to be serotype-specific and a major antigenic determinant. Objective: It is important to know the circulating serotypes in a particular geographical location for effective control of the disease. The present study unravels the molecular evolution of the circulating BTV serotypes during 2014–2018 in Telangana and Andhra Pradesh states of India. Methods: Multiple sequence alignment with available BTV serotypes in GenBank and phylogenetic analysis were performed for the partial VP2 sequences of major circulating BTV serotypes during the study period. Results: The multiple sequence alignment of circulating serotypes with respective reference isolates revealed variations in antigenic VP2. The phylogenetic analysis revealed that the major circulating serotypes were grouped into eastern topotypes (BTV-1, BTV-2, BTV-4, and BTV-16) and Western topotypes (BTV-5, BTV-12, and BTV-24). Conclusion: Our study strengthens the need for development of an effective vaccine, which can induce the immune response for a range of serotypes within and in between topotypes.
Intervirology

中文翻译：

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2014-2018 年从印度泰伦加纳邦和安得拉邦分离的主要循环蓝舌病毒血清型的基于 VP2 基因的分子进化模式


简介：蓝舌病是由多种血清型的蓝舌病毒（BTV）引起的一种重要的经济牲畜病毒性疾病。它属于呼肠弧病毒科、Sedoreovirinae 亚科的环状病毒属。 BTV 的基因组是 10 段 dsRNA，编码 7 种结构蛋白和 4 种非结构蛋白，其中 VP2 据报道具有血清型特异性，是主要的抗原决定簇。目的：了解特定地理位置的流行血清型对于有效控制该疾病非常重要。本研究揭示了 2014 年至 2018 年印度泰伦加纳邦和安得拉邦流通的 BTV 血清型的分子进化。方法：对研究期间主要流行的BTV血清型的部分VP2序列与GenBank中可用的BTV血清型进行多重序列比对和系统发育分析。结果：循环血清型与相应参考分离株的多重序列比对揭示了抗原性 VP2 的变异。系统发育分析显示，主要流行血清型分为东部拓扑型（BTV-1、BTV-2、BTV-4 和 BTV-16）和西部拓扑型（BTV-5、BTV-12 和 BTV-24）。结论：我们的研究强调了开发有效疫苗的必要性，这种疫苗可以诱导针对拓扑型内和拓扑型之间的一系列血清型的免疫反应。
 病毒间学