Introduction: Biological rhythm is inextricably linked to the physiological mechanisms of allergic diseases, but the exact mechanisms are still poorly understood. Clinical studies have reported rhythmic fluctuations in allergic diseases. The search for natural and harmless active ingredients based on biological rhythm with which to regulate allergic diseases is essential for the control of food allergy. Methods: In this study, mice were treated at different time points to determine the link between the severity of allergic reactions and the circadian clock genes. The mice were treated with lentinan, either continuously or discontinuously, to assess their clinical symptoms, vascular permeability, immune cells, cytokines, and clock genes. Specifically, rat basophilic leukemia (RBL-2H3) cells were treated with lentinan and the rhythmic changes of cell degranulation were measured. Results: The results in different models showed that the allergic reactions in mice treated at different time points were significantly different and thus related to fluctuations in biological rhythm. Treatment with lentinan was found to reduce the amplitude of changes in the clock genes, such as the activation of Per and Cry proteins in allergic mice, as well as to regulate biological rhythm in cells, inhibit the activation of Th2 cells, and alleviate allergic reactions. Furthermore, lentinan changed the rhythm of degranulation in RBL-2H3 cells. Conclusion: Lentinan was, therefore, determined to successfully alleviate allergic reactions by reducing the amplitude of changes in the body’s biological rhythm, inhibiting the activation of Th2 cells, and affecting the immune microenvironment.
Int Arch Allergy Immunol

中文翻译：

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香菇多糖通过降低生物节律变化幅度抑制过敏小鼠 Th2 细胞的激活

简介：生物节律与过敏性疾病的生理机制密不可分，但其确切机制仍知之甚少。临床研究报告了过敏性疾病的节律性波动。寻找基于生物节律的天然无害活性成分来调节过敏性疾病对于控制食物过敏至关重要。方法：在这项研究中，小鼠在不同的时间点接受治疗，以确定过敏反应的严重程度与生物钟基因之间的联系。用香菇多糖连续或不连续地治疗小鼠，以评估它们的临床症状、血管通透性、免疫细胞、细胞因子和时钟基因。具体而言，用香菇多糖处理大鼠嗜碱性白血病（RBL-2H3）细胞并测量细胞脱颗粒的节律性变化。结果：不同模型的结果表明，在不同时间点治疗的小鼠的过敏反应存在显着差异，因此与生物节律的波动有关。发现用香菇多糖处理可降低时钟基因的变化幅度，如过敏小鼠的 Per 和 Cry 蛋白的激活，以及调节细胞内的生物节律，抑制 Th2 细胞的激活，减轻过敏反应. 此外，香菇多糖改变了 RBL-2H3 细胞脱颗粒的节奏。结论：因此，香菇多糖通过降低机体生物节律变化幅度、抑制Th2细胞活化、影响免疫微环境，成功缓解过敏反应。
Int Arch 过敏免疫