Effect of extended storage on egg quality, embryo mortality and hatchability in FUNAAB-ɑ chickens was determined. Hatchable eggs (n = 288; weighing 53.2 ± 4.67 g) collected from a flock of FUNAAB-ɑ layer breeder hens aged 32 weeks were stored in egg tray with broad end up under 16 ± 1.5°C for either 0, 4, 8, 12, 16 or 20 d. Before incubation, eight eggs from each group were evaluated for internal and external quality traits. Remaining eggs were set in an incubator and transferred into hatcher on embryonic day 18. Data collected were subjected to one-way analysis of variance. Egg weight loss (EWL; p < .001), surface area (p < .001), yolk diameter (p < .001), inner and outer blastoderm diameters (p < .05) and dead in germ (DIG; p < .001) increased with storage duration while yolk height (p < .001), yolk index (p < .001), albumen weight (p < .05), albumen height (p < .05), albumen index (p < .01), Haugh's unit (HU; p < .05), fertility (p < .001), hatchability of set (HATCHS; p < .001) and fertile eggs (p < .05) decreased. Weight losses of 0, 1.2, 2.2, 3.4, 4.6 and 6.1% were recorded in egg stored for 0, 4, 8, 12, 16 and 20 days respectively. Eggs stored beyond 8 days exhibited higher DIG and lower HATCHS. Shell percentage in 4 days storage (11.4%) was lower (p < .05) than in 16 days storage (13.4%). Shell thickness was similar in eggs stored for 0 to 12 days, but 8 days storage (0.60 mm) had thinner (p < .01) shell than day 16 (0.71 mm) and day 20 (0.73 mm) storage. Internal quality unit (IQU) was higher (p < .05) in fresh eggs (180.4) than in 12 days (167.8) and 20 days (167.8) stored eggs. Extended storage of FUNAAB-ɑ eggs caused EWL, surface area shrinkage, lowered HU and IQU, loss of yolk and albumen quality, increased blastoderm diameters and DIG, and decreased egg fertility and HATCHS from day 8 forward. Storing FUNAAB-ɑ eggs beyond 8 days reduced quality parameters; therefore, other mitigating factors are recommended when storing beyond 8 days.

中文翻译：

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贮藏时间对FUNAAB-ɑ鸡卵质量、胚胎死亡率和孵化率的影响

确定了延长储存对 FUNAAB-ɑ 鸡的蛋质量、胚胎死亡率和孵化率的影响。 从一群32 周龄的FUNAAB-ɑ蛋鸡种鸡中收集的可孵化蛋（n = 288；重 53.2 ± 4.67 g）被储存在蛋托中，宽端在 16 ± 1.5°C 下储存0、4、8、 12、16 或 20 天。在孵化前，对每组 8 个鸡蛋的内部和外部质量特征进行了评估。剩余的种蛋放置在孵化器中，并在胚胎第 18 天转移到孵化器中。收集的数据进行单向方差分析。鸡蛋重量损失 (EWL; p  < .001)、表面积 ( p  < .001)、蛋黄直径 ( p  < .001)、内外胚盘直径 (p  < .05) 和胚芽死亡 (DIG; p  < .001) 随着储存时间的延长而增加，而蛋黄高度 ( p  < .001)、蛋黄指数 ( p  < .001)、蛋白重量 ( p  < .05)、蛋白高度 ( p  < .05)、蛋白指数 ( p  < .01)、哈夫单位 (HU; p  < .05)、繁殖力 ( p  < .001)、孵化率 (HATCHS; p  < .001) 和受精卵( p  < .05) 下降。在储存 0、4、8、12、16 和 20 天的鸡蛋中分别记录了 0、1.2、2.2、3.4、4.6 和 6.1% 的重量损失。储存超过 8 天的鸡蛋表现出更高的 DIG 和更低的孵化率。4 天储存中的壳百分比 (11.p  < .05) 高于 16 天的储存 (13.4%)。储存 0 至 12 天的鸡蛋壳厚度相似，但储存 8 天 (0.60 毫米 ) 的蛋壳比储存 16 天 (0.71 毫米) 和第 20 天 (0.73 毫米) 的蛋壳更薄 ( p < .01)。 新鲜鸡蛋的内部质量单位 (IQU) (180.4) 高于储存 12 天 (167.8) 和 20 天 (167.8) 的鸡蛋( p < .05)。FUNAAB-ɑ 鸡蛋的长期储存导致 EWL、表面积缩小、HU 和 IQU 降低、蛋黄和蛋白质量下降、胚盘直径和 DIG 增加，以及从第 8 天开始降低鸡蛋的生育能力和孵化率。将 FUNAAB-ɑ 鸡蛋储存超过 8 天会降低质量参数；因此，当储存超过 8 天时，建议使用其他缓解因素。