Forensic application and evaluation of a commercially available pregabalin immunoassay test in serum on an Olympus AU480,Drug Testing and Analysis

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Forensic application and evaluation of a commercially available pregabalin immunoassay test in serum on an Olympus AU480
Drug Testing and Analysis ( IF 2.6 ) Pub Date : 2020-12-29 , DOI: 10.1002/dta.2994
Cornelius Hess ₁ , Sandra Zeidler ₁ , Joerg Roehrich ₁

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Misuse of pregabalin and its forensic relevance is steadily increasing. The aim of this study was to evaluate the usability of the commercially available ARKTM Pregabalin II Assay (ARK Diagnostics) for serum analysis of forensic samples. Overall, 156 samples were tested by both the immunoassay and a validated liquid chromatographic–tandem mass spectrometric (LC-MS/MS) method. Sensitivity was 100%, and specificity was 98.7% (n = 79 positive cases confirmed by LC-MS/MS in a range of 380–37,000 ng/mL). A good correlation (R² = 0.73) could also be shown between quantitative immunoassay and LC-MS/MS results. In conclusion, the assay shows excellent reliability for screening of forensic serum samples.

中文翻译：

奥林巴斯 AU480 血清中市售普瑞巴林免疫分析测试的法医应用和评估

普瑞巴林的滥用及其法医相关性正在稳步增加。本研究的目的是评估市售的 ARKTM Pregabalin II Assay（ARK Diagnostics）在法医样本血清分析中的可用性。总体而言，156 个样品通过免疫测定和经过验证的液相色谱-串联质谱 (LC-MS/MS) 方法进行了测试。灵敏度为 100%，特异性为 98.7%（n = 79 例阳性病例，经 LC-MS/MS 确认，范围为 380-37,000 ng/mL）。定量免疫测定和 LC-MS/MS 结果之间也显示出良好的相关性 ( R ² = 0.73)。总之，该测定显示出法医血清样本筛选的出色可靠性。

更新日期：2020-12-29

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