PG1659 functions as anti‐sigma factor to extracytoplasmic function sigma factor RpoE in Porphyromonas gingivalis W83,Molecular Oral Microbiology

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PG1659 functions as anti‐sigma factor to extracytoplasmic function sigma factor RpoE in Porphyromonas gingivalis W83
Molecular Oral Microbiology ( IF 2.8 ) Pub Date : 2020-12-29 , DOI: 10.1111/omi.12329
Yuetan Dou ₁ , Hiel Rutanhira ₁ , Norbert Schormann ₂ , Champion Deivanayagam ₂ , Hansel M Fletcher ₁

Affiliation

Anti‐sigma factors play a critical role in regulating the expression of sigma factors in response to environmental stress signals. PG1659 is cotranscribed with an upstream gene PG1660 (rpoE), which encodes for a sigma factor that plays an important role in oxidative stress resistance and the virulence regulatory network of P. gingivalis. PG1659, which is annotated as a hypothetical gene, is evaluated in this study. PG1659, composed of 130 amino acids, is predicted to be transmembrane protein with a single calcium (Ca²⁺) binding site. In P. gingivalis FLL358 (ΔPG1659::ermF), the rpoE gene was highly upregulated compared to the wild‐type W83 strain. RpoE‐induced genes were also upregulated in the PG1659‐defective isogenic mutant. Both protein‐protein pull‐down and bacterial two‐hybrid assays revealed that the PG1659 protein could interact with/bind RpoE. The N‐terminal domain of PG1659, representing the cytoplasmic fragment of the protein, is critical for interaction with RpoE. In the presence of PG1659, the initiation of transcription by the RpoE sigma factor was inhibited. Taken together, our data suggest that PG1659 is an anti‐sigma factor which plays an important regulatory role in the modulation of the sigma factor RpoE in P. gingivalis.

中文翻译：

PG1659 在牙龈卟啉单胞菌 W83 中作为胞质外功能 σ 因子 RpoE 的抗 σ 因子发挥作用

抗 sigma 因子在调节 sigma 因子响应环境应激信号的表达中起关键作用。PG1659与上游基因PG1660 ( rpoE ) 共同转录，该基因编码在抗氧化应激和P的毒力调节网络中起重要作用的 sigma 因子。牙龈炎。在本研究中评估了被注释为假设基因的PG1659 。PG1659 由 130 个氨基酸组成，预计是具有单个钙 (Ca ²⁺ ) 结合位点的跨膜蛋白。在P中。牙龈菌FLL358 (Δ PG1659 ::ermF )，与野生型 W83 菌株相比， rpoE基因高度上调。RpoE 诱导的基因也在PG1659缺陷型等基因突变体中上调。蛋白质-蛋白质下拉和细菌双杂交试验均显示 PG1659 蛋白可以与 RpoE 相互作用/结合。PG1659 的 N 端结构域代表蛋白质的细胞质片段，对于与 RpoE 的相互作用至关重要。在 PG1659 存在下，RpoE σ 因子的转录起始被抑制。总之，我们的数据表明 PG1659 是一种抗 sigma 因子，在P中 sigma 因子 RpoE 的调节中起重要调节作用。牙龈炎。

更新日期：2021-03-08

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