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Immune function changes of the IDPN induced Tourette Syndrome rat model
International Journal of Developmental Neuroscience ( IF 1.7 ) Pub Date : 2021-01-12 , DOI: 10.1002/jdn.10085
Xiumei Liu 1 , Xueming Wang 2 , Aihua Cao 3 , Xiaoling Zhang 4
Affiliation  

There may be immunologic alternations during Tourette syndrome (TS) development. This study aimed to determine the immune function changes in different aspects (spleen or thymus index, plasma cytokines, and T cell) in an 3,3'-iminodipropionitrile (IDPN)-induced rat model of TS. Male Sprague-Dawley rats were assigned to control and TS groups. The control group received intraperitoneal infections of normal saline (5 ml/kg/day), and the TS rats were injected with IDPN (150 mg/kg/day). The spleen and thymus indices were calculated. The expression of anti-inflammatory cytokines and inflammatory cytokines TNF-α, in peripheral blood were measured by ELISA and Western blotting. The proportion of CD3+, CD4+, CD8+, Treg, Th1 and Th2 cells were determined by fluorescence-activated cell sorting analysis. After one week of IDPN treatment, TS rats had decreased spleen and thymus weights versus control. The plasma levels of IL-4, IL-10, IL-12, IFN-γ, and TNF-α were significantly increased, while no significant difference in TGF-β was found. Flow cytometry analysis demonstrated that TS rats had significantly reduced CD3+ and CD4+ cells in spleen, without any change in the proportion of CD8+ cells. Further, the ratio of Treg cells (CD4+/CD25+/FoxP3+) was decreased in TS rats; simultaneously, Th1 cells (CD4+/IFN-γ+) and Th2 cells (CD4+/IL4+) were dramatically increased. Together, IDPN can trigger immune dysfunction through impairment of matured Th cells, in particular for the Treg subset.

中文翻译:

IDPN诱导Tourette综合征大鼠模型免疫功能变化

在图雷特综合征 (TS) 发展过程中可能存在免疫学改变。本研究旨在确定 3,3'-亚氨基二丙腈 (IDPN) 诱导的 TS 大鼠模型中不同方面(脾脏或胸腺指数、血浆细胞因子和 T 细胞)的免疫功能变化。雄性 Sprague-Dawley 大鼠被分配到对照组和 TS 组。对照组腹腔注射生理盐水(5 ml/kg/day),TS大鼠注射IDPN(150 mg/kg/day)。计算脾和胸腺指数。通过ELISA和Western印迹测定外周血中抗炎细胞因子和炎性细胞因子TNF-α的表达。CD3+、CD4+、CD8+、Treg、Th1和Th2细胞的比例通过荧光激活细胞分选分析确定。经过一周的 IDPN 治疗,与对照相比,TS 大鼠的脾脏和胸腺重量降低。血浆IL-4、IL-10、IL-12、IFN-γ和TNF-α水平显着升高,而TGF-β无显着差异。流式细胞术分析表明,TS大鼠脾脏CD3+和CD4+细胞显着减少,CD8+细胞比例没有任何变化。此外,TS大鼠中Treg细胞的比例(CD4+/CD25+/FoxP3+)降低;同时,Th1细胞(CD4+/IFN-γ+)和Th2细胞(CD4+/IL4+)显着增加。总之,IDPN 可以通过损伤成熟的 Th 细胞,特别是 Treg 亚群来引发免疫功能障碍。流式细胞术分析表明,TS大鼠脾脏CD3+和CD4+细胞显着减少,CD8+细胞比例没有任何变化。此外,TS大鼠中Treg细胞的比例(CD4+/CD25+/FoxP3+)降低;同时,Th1细胞(CD4+/IFN-γ+)和Th2细胞(CD4+/IL4+)显着增加。总之,IDPN 可以通过损伤成熟的 Th 细胞,特别是 Treg 亚群来引发免疫功能障碍。流式细胞术分析表明,TS大鼠脾脏CD3+和CD4+细胞显着减少,CD8+细胞比例没有任何变化。此外,TS大鼠中Treg细胞的比例(CD4+/CD25+/FoxP3+)降低;同时,Th1细胞(CD4+/IFN-γ+)和Th2细胞(CD4+/IL4+)显着增加。总之,IDPN 可以通过损伤成熟的 Th 细胞,特别是 Treg 亚群来引发免疫功能障碍。
更新日期:2021-01-12
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