Several forms of bacterial exopolysaccharides (EPSs) have been reported as having industrial and medical benefits. Previously, our group had achieved a high yield of EPS production (8.6 gm/L) from Enterobacter sp. isolated from the marine environment of Haqel Beach, in the Tabuk region of Saudi Arabia, by optimizing its culture conditions. Then, the strain selected was identified using conventional methods and confirmed by molecular characterization that used 16S Ribosomal RNA (16S rRNA) gene sequencing. In the present study, we determined the composition of this EPS and evaluated its anticoagulation, fibrinolytic, antimicrobial, and prebiotic activities. We determined the monosaccharide moieties of this EPS using acid hydrolysis by qualitative and quantitative paper chromatography (PC). The monosaccharide composition of this EPS was almost 25% glucose, 25% galactose, 40% fucose, in addition to 10% uronic acid, with traces of fructose. Sulfation modification was applied to the EPS produced. The biological and microbiological (anticoagulation, fibrinolytic, antimicrobial, and prebiotic) activities of both the native and sulfated EPS were investigated and both exhibited higher antibacterial activity against Staphyllococcus aureus and Escherichia coli, although neither form displayed antifungal activity against Candida albicans. The minimum inhibitory concentration (MIC) of the native and sulfated EPS that sustained the highest bacterial inhibition zone was 15 mg/dl. No prebiotic activities were recorded for either EPS. In conclusion, the biopolymer produced has significant biological activities that require further adaptation for practical and industrial applications.

据报道，几种形式的细菌胞外多糖（EPS）具有工业和医学优势。以前，我们的团队已经从肠杆菌中获得了高产量的EPS产量（8.6 gm / L）sp。通过优化养殖条件，与沙特阿拉伯塔布克地区哈克尔海滩的海洋环境隔离。然后，使用常规方法鉴定所选菌株，并通过使用16S核糖体RNA（16S rRNA）基因测序的分子表征进行确认。在本研究中，我们确定了这种EPS的成分，并评估了其抗凝，纤溶，抗菌和益生元的活性。我们通过定性和定量纸色谱法（PC）进行酸水解，确定了该EPS的单糖部分。除了10％的糖醛酸外，这种EPS的单糖成分几乎是25％葡萄糖，25％半乳糖，40％果糖和微量的果糖。将硫酸化改性剂应用于生产的EPS。生物和微生物学（抗凝，金黄色葡萄球菌和大肠埃希菌，尽管两种形式都没有显示对白念珠菌的抗真菌活性。维持最高细菌抑制区的天然和硫酸化EPS的最小抑菌浓度（MIC）为15 mg / dl。两种EPS均未记录益生元活性。总之，所生产的生物聚合物具有重要的生物学活性，需要进一步适应实际和工业应用。