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Ureaplasma diversum and Ureaplasma sp. in nasal cavity of pigs: Distribution among herds and individual frequency based on pooled samples
Revista Argentina de Microbiología ( IF 1.9 ) Pub Date : 2020-12-30 , DOI: 10.1016/j.ram.2020.11.002
Jimena Seitz 1 , Gastón Vicharelli 1 , Virginia García 1 , Claudina Vissio 2 , Abel Estanguet 1 , Julián Parada 2 , Alicia Carranza 1 , Gabriel Di Cola 1 , Pablo Tamiozzo 1
Affiliation  

The role of Ureaplasma diversum in the porcine respiratory disease complex (PRDC), its circulation among herds and prevalence in live pigs is unknown; thus, the objectives of this study were: to determine the presence of U. diversum in indoor intensive pig herds and to determine the individual frequency of pigs with U. diversum from pooled samples. A cross sectional study was carried out in 16 indoor intensive herds from Córdoba and La Pampa provinces, collecting eight nasal swabs specimens that were further processed by two pools of four specimens each by a PCR targeting a fragment of 16S ribosomal RNA gene. Four PCR products were sequenced and aligned against data bases. U. diversumUreaplasma sp. were detected in 56.3% of the analyzed herds, with 16.5% (95% CI 8.2–30.8) of positive pigs. It was concluded that U. diversumUreaplasma sp. are present in the nasal cavity of live pigs, being widely distributed among herds.



中文翻译:

Ureaplasma diversum 和 Ureaplasma sp。在猪的鼻腔中:基于汇总样本的猪群分布和个体频率

的作用解diversum在猪呼吸道疾病综合征(PRDC),其在生猪存栏率和患病率之间的循环是未知的; 因此,本研究的目的是:确定室内集约化猪群中是否存在鲣鱼,并从汇集的样本中确定具有鲟鱼的猪的个体频率。对来自科尔多瓦省和拉潘帕省的 16 个室内密集猪群进行了横断面研究,收集了 8 个鼻拭子样本,这些样本由两个由四个样本组成的集合通过针对 16S 核糖体 RNA 基因片段的 PCR 进一步处理。对四种 PCR 产物进行测序并与数据库比对。U. diversum脲原体sp. 在 56.3% 的分析猪群中检测到了 16.5% (95% CI 8.2–30.8) 的阳性猪。结论是U. diversum - Ureaplasma sp。存在于活猪的鼻腔中,广泛分布于猪群中。

更新日期:2020-12-30
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