当前位置: X-MOL 学术Biomater. Adv. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Self-assembled guanosine-hydrogels for drug-delivery application: Structural and mechanical characterization, methylene blue loading and controlled release
Biomaterials Advances ( IF 5.5 ) Pub Date : 2020-12-30 , DOI: 10.1016/j.msec.2020.111834
Juliana S. Yoneda , Daniele R. de Araujo , Fiorenza Sella , Gabriel R. Liguori , Tácia T.A. Liguori , Luiz Felipe P. Moreira , Francesco Spinozzi , Paolo Mariani , Rosangela Itri

It is known that guanosine derivatives (G) self-assemble in water forming long, flexible, and interacting aggregates (the so-called G-quadruplexes): by modulating the quadruplex charges, e.g. simply using a mixture of guanosine 5′-monophosphate (GMP) and guanosine (Gua), multi-responsive, self-healing hydrogels can be obtained. In this paper, the potential application of G-hydrogels as drug delivery systems has been assessed. Hydrogels were prepared at different Gua:GMP molar ratios. The photosensitizer Methylene Blue and the pro-apoptotic protein cytochrome C were used as cargo molecules. Small angle x-ray scattering and atomic force microscopy experiments confirmed the presence of G-quadruplexes disposed in swollen matrices with different mesh-sizes. Rheology measurements showed that the Gua:GMP molar ratio leads to specific drug release mechanisms, as the gel strength is finely tuned by electrostatic repulsion and van der Waals attraction between G-quadruplexes. Noteworthy, the gel cohesion and the drug release were pH responsive. Swelling, self-healing and cell viability features were also investigated: the results qualify the Gua:GMP hydrogel as an excellent biomaterial that can entrap and deliver key biomolecules in a sustained and responsive release manner.



中文翻译:

用于药物递送应用的自组装鸟苷水凝胶:结构和机械表征,亚甲蓝负载量和控释

已知鸟苷衍生物(G)在水中自组装形成长的,柔性的和相互作用的聚集体(所谓的G-四链体):通过调节四链体电荷,例如简单地使用鸟苷5'-单磷酸酯的混合物( GMP)和鸟苷(Gua),可以获得多反应性,自愈的水凝胶。在本文中,已经评估了G-水凝胶作为药物递送系统的潜在应用。以不同的Gua:GMP摩尔比制备水凝胶。光敏剂亚甲基蓝和促凋亡蛋白细胞色素C被用作货物分子。小角度X射线散射和原子力显微镜实验证实,存在于具有不同筛孔尺寸的溶胀基质中的G四联体存在。流变学测量表明,刮a:GMP摩尔比可导致特定的药物释放机制,因为凝胶强度可通过G-四链体之间的静电排斥和范德华力吸引进行微调。值得注意的是,凝胶内聚力和药物释放是pH响应的。还研究了肿胀,自我修复和细胞生存能力:结果证明Gua:GMP水凝胶是一种优秀的生物材料,可以持续和响应性释放的方式截留和递送关键生物分子。

更新日期:2021-01-18
down
wechat
bug