Two interleukin (IL)-17 N genes (CcIL-17Na and b) present on different linkage groups were identified in the common carp (Cyprinus carpio) genome and confirmed by polymerase chain reaction (PCR) and real time (RT)-PCR in this experiment. Synteny analysis revealed that IL-17 N is transcribed by the complement sequence of TOP3B's intron 2. It is flanked by SDF2L and PPM1F in all fish studied to date, except fugu (Takifugu rubripes). The open reading frames of the two CcIL-17Ns are 411 base pairs long and encode 136 amino acids. The amino acid identity/similarity between CcIL-17Na and b is 91.2%/97.1%. The CcIL-17Ns share identity (46.8–90.4%) with their orthologs from other teleosts. Identities/similarities to other members of the IL-17 family in common carp were low at 21.4–30.2%/31.4–51.4%. In the phylogenetic tree, IL-17Ns from spotted gar (Lepisosteus oculatus, the ancestor of teleosts) and coelacanth (Latimeria chalumnae, the ancestor of tetrapods) were grouped within the same branch with a high bootstrap value of 97%, which indicates that IL-17 N is an ancient and conserved gene. Quantitative RT-PCR results showed that CcIL-17Ns were most highly expressed in the brain of healthy individuals. The expression in brain was significantly induced at 6 h post Aeromonas hydrophila infection; at 1 day post infection, expression in liver, muscle, skin, spleen, and head kidney was up-regulated. In addition, the upregulated expression of proinflammatory cytokines IL-1β, IFN-γ, IL-6, chemokine CCL20, NF - κ B and TRAF6 in kidney tissue by ccIL-17 N recombinant protein also indicate that IL-17 N can promote inflammation through NF-κB pathway and induce the expression of chemokines and inflammatory factors.

在普通鲤鱼（Cyprinus carpio）基因组中鉴定出两个存在于不同连锁群上的白细胞介素（IL）-17 N基因（CcIL-17Na和b），并通过聚合酶链反应（PCR）和实时（RT）PCR进行了证实。这个实验。同源性分析显示，IL-17 N被TOP3B内含子2的互补序列转录。在迄今研究的所有鱼类中，除河豚（Takifugu rubripes）之外，其侧翼均为SDF2L和PPM1F 。两个CcIL-17N的开放阅读框长411个碱基对，编码136个氨基酸。CcIL-17Na和b之间的氨基酸同一性/相似性是91.2％/ 97.1％。CcIL-17N与其他硬骨鱼的直系同源物具有相同的身份（46.8–90.4％）。与鲤鱼中IL-17家族其他成员的同一性/相似性较低，分别为21.4–30.2％/ 31.4–51.4％。在系统发育树中，斑点（硬骨鱼类的祖先Lepisosteus oculatus）和腔棘鱼（四足动物的祖先Latimeria chalumnae）的IL-17Ns被归入同一分支内，自举值高达97％，这表明IL -17 N是古老且保守的基因。定量RT-PCR结果表明，CcIL- 17N在健康个体的大脑中表达最高。嗜水气单胞菌感染后6小时大脑中的表达明显升高感染; 感染后1天，肝脏，肌肉，皮肤，脾脏和头部肾脏中的表达上调。此外，ccIL-17 N重组蛋白在肾脏组织中促炎细胞因子IL-1β，IFN-γ，IL-6，趋化因子CCL20，NF-κB和TRAF6的表达上调也表明IL-17N可以促进炎症通过NF-κB途径诱导趋化因子和炎症因子的表达。