Background

H₂S is proved to be functioning as a signaling molecule in an array of physiological processes in the plant and animal kingdom. However, the H₂S synthesis pathway and the responses to cold conditions remain unclear in postharvest mushroom.

Results

The biosynthesis of H₂S in the Agaricus bisporus mushroom tissues exhibited an increasing tendency during postharvest storage and was significantly triggered by cold treatment. The cystathionine γ-lyase (AbCSE) and cystathionine β-synthase (AbCBS) genes were cloned and proved responsible for H₂S biosynthesis. Furthermore, transcriptional and posttranscriptional regulation of AbCSE and AbCBS were crucial for the enzyme activities and subsequent H₂S levels. However, the AbMST was not involved in this process. Moreover, the AbCSE and AbCBS genes displayed low identity to the characterized genes, but typical catalytic domains, activity sites, subunit interface sites, and cofactor binding sites were conserved in the respective protein sequences, as revealed by molecular modeling and docking study. The potential transcription factors responsible for the H₂S biosynthesis in cold conditions were also provided.

Conclusions

The H₂S biosynthetic pathway in postharvest mushroom was unique and distinct to that of other horticultural products.

How to cite: Liu Y, Pei Y, Zhu D, et al. H₂S is synthesized via CBS/CSE pathway and triggered by cold conditions during Agaricus bisporus storage. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2020.12.004

中文翻译：

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H ₂ S是通过CBS / CSE途径合成的，并在双孢蘑菇储存期间受寒冷条件触发

背景

H ₂ S被证明在动植物界的一系列生理过程中起着信号分子的作用。但是，在采后蘑菇中，H ₂ S的合成途径和对寒冷条件的反应仍不清楚。

结果

双孢蘑菇的蘑菇组织中H ₂ S的生物合成在收获后的贮藏过程中显示出增加的趋势，并且通过冷处理显着触发。克隆了胱硫醚γ-裂合酶（AbCSE）和胱硫醚β-合酶（AbCBS）基因，并证明其负责H ₂ S的生物合成。此外，AbCSE和AbCBS的转录和转录后调控对于酶活性和随后的H ₂ S水平至关重要。但是，AbMST没有参与此过程。此外，AbCSE和AbCBS基因显示与特征基因的同一性低，但是典型的催化结构域，活性位点，亚基界面位点和辅因子结合位点在各自的蛋白质序列中均保守，如分子模型和对接研究所揭示。还提供了在寒冷条件下负责H ₂ S生物合成的潜在转录因子。

结论

采后蘑菇中的H ₂ S生物合成途径是独特的，与其他园艺产品截然不同。

引用方法：刘Y，裴Y，朱D等。H ₂ S是通过CBS / CSE途径合成的，并在双孢蘑菇储存过程中受寒冷条件触发。电子J生物技术2021; 50。https://doi.org/10.1016/j.ejbt.2020.12.004