当前位置：
X-MOL 学术
›
Environ. Sci. Technol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Shifts in a Phenanthrene-Degrading Microbial Community are Driven by Carbohydrate Metabolism Selection in a Ryegrass Rhizosphere
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-12-28 , DOI: 10.1021/acs.est.0c04951 Longfei Jiang 1 , Chunling Luo 1, 2 , Dayi Zhang 3 , Mengke Song 2 , Weiping Mei 2 , Yingtao Sun 1 , Gan Zhang 1
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-12-28 , DOI: 10.1021/acs.est.0c04951 Longfei Jiang 1 , Chunling Luo 1, 2 , Dayi Zhang 3 , Mengke Song 2 , Weiping Mei 2 , Yingtao Sun 1 , Gan Zhang 1
Affiliation
|
Plants usually promote pollutant bioremediation by several mechanisms including modifying the diversity of functional microbial species. However, conflicting results are reported that root exudates have no effects or negative effects on organic pollutant degradation. In this study, we investigated the roles of ryegrass in phenanthrene degradation in soils using DNA stable isotope probing (SIP) and metagenomics to reveal a potential explanation for conflicting results among phytoremediation studies. Phenanthrene biodegradation efficiency was improved by 8% after 14 days of cultivation. Twelve and ten operational taxonomic units (OTUs) were identified as active phenanthrene degraders in non-rhizosphere and rhizosphere soils, respectively. The active phenanthrene degraders exhibited higher average phylogenetic distances in rhizosphere soils (0.33) than non-rhizosphere soils (0.26). The Ka/Ks values (the ratio of nonsynonymous to synonymous substitutions) were about 10.37% higher in the rhizosphere treatment among >90% of all key carbohydrate metabolism-related genes, implying that ryegrass may be an important driver of microbial community variation in the rhizosphere by relieving the carbohydrate metabolism pressure and improving the survival ability of r-strategy microbes. Most Ka/Ks values of root-exudate-related metabolism genes exhibited little change, except for fumarate hydratase that increased 13-fold in the rhizosphere compared to that in the non-rhizosphere treatment. The Ka/Ks values of less than 50% phenanthrene-degradation-related genes were affected, 30% of which increased and 70% behaved oppositely. Genes with altered Ka/Ks values had a low percentage and followed an inconsistent changing tendency, indicating that phenanthrene and its metabolites are not major factors influencing the active degraders. These results suggested the importance of carbohydrate metabolism, especially fumaric acid, in rhizosphere community shift, and hinted at a new hypothesis that the rhizosphere effect on phenanthrene degradation efficiency depends on the existence of active degraders that have competitive advantages in carbohydrate and fumaric acid metabolism.
中文翻译：
在黑麦草根际中碳水化合物代谢选择驱动着菲降解微生物群落的转变。
植物通常通过几种机制来促进污染物的生物修复，包括改变功能性微生物物种的多样性。但是，据报矛盾的结果是，根系分泌物对有机污染物的降解没有影响或没有负面影响。在这项研究中，我们使用DNA稳定同位素探测（SIP）和宏基因组学研究了黑麦草在土壤中菲的降解中的作用，从而揭示了植物修复研究中相互矛盾的结果的潜在解释。培养14天后，菲的生物降解效率提高了8％。在非根际土壤和根际土壤中，分别有十二个和十个可操作的生物分类单位（OTU）被确定为活性菲的降解物。活性菲降解物在根际土壤中表现出更高的平均系统发生距离（0。33）比非根际土壤（0.26）。的在大于90％的所有关键的碳水化合物代谢相关基因中，根际处理中的K a / K s值（非同义替换与同义替换的比率）高约10.37％，这表明黑麦草可能是导致微生物群落变异的重要驱动力。通过减轻碳水化合物的代谢压力和提高r策略微生物的生存能力来根际。根分泌物相关代谢基因的大多数K a / K s值几乎没有变化，除了富马酸酯水合酶与非根际处理相比增加了13倍。所述ķ一个/ ķ小号低于50％的菲降解相关基因的值受到影响，其中30％升高，而70％表现相反。具有改变的K a / K s值的基因具有较低的百分比，并且遵循不一致的变化趋势，这表明菲及其代谢物不是影响活性降解物的主要因素。这些结果表明碳水化合物代谢，尤其是富马酸在根际群落转移中的重要性，并暗示了一个新的假说，即根际对菲降解效率的影响取决于在碳水化合物和富马酸代谢中具有竞争优势的活性降解剂的存在。
更新日期：2021-01-19
中文翻译：
在黑麦草根际中碳水化合物代谢选择驱动着菲降解微生物群落的转变。
植物通常通过几种机制来促进污染物的生物修复，包括改变功能性微生物物种的多样性。但是，据报矛盾的结果是，根系分泌物对有机污染物的降解没有影响或没有负面影响。在这项研究中，我们使用DNA稳定同位素探测（SIP）和宏基因组学研究了黑麦草在土壤中菲的降解中的作用，从而揭示了植物修复研究中相互矛盾的结果的潜在解释。培养14天后，菲的生物降解效率提高了8％。在非根际土壤和根际土壤中，分别有十二个和十个可操作的生物分类单位（OTU）被确定为活性菲的降解物。活性菲降解物在根际土壤中表现出更高的平均系统发生距离（0。33）比非根际土壤（0.26）。的在大于90％的所有关键的碳水化合物代谢相关基因中，根际处理中的K a / K s值（非同义替换与同义替换的比率）高约10.37％，这表明黑麦草可能是导致微生物群落变异的重要驱动力。通过减轻碳水化合物的代谢压力和提高r策略微生物的生存能力来根际。根分泌物相关代谢基因的大多数K a / K s值几乎没有变化，除了富马酸酯水合酶与非根际处理相比增加了13倍。所述ķ一个/ ķ小号低于50％的菲降解相关基因的值受到影响，其中30％升高，而70％表现相反。具有改变的K a / K s值的基因具有较低的百分比，并且遵循不一致的变化趋势，这表明菲及其代谢物不是影响活性降解物的主要因素。这些结果表明碳水化合物代谢，尤其是富马酸在根际群落转移中的重要性，并暗示了一个新的假说，即根际对菲降解效率的影响取决于在碳水化合物和富马酸代谢中具有竞争优势的活性降解剂的存在。
京公网安备 11010802027423号