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Taylor Dispersion Analysis Coupled to Inductively Coupled Plasma-Mass Spectrometry for Ultrasmall Nanoparticle Size Measurement: From Drug Product to Biological Media Studies
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-12-29 , DOI: 10.1021/acs.analchem.0c03988 Lucie Labied 1, 2 , Paul Rocchi 2, 3 , Tristan Doussineau 3 , Jérôme Randon 1 , Olivier Tillement 2 , François Lux 2, 4 , Agnès Hagège 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-12-29 , DOI: 10.1021/acs.analchem.0c03988 Lucie Labied 1, 2 , Paul Rocchi 2, 3 , Tristan Doussineau 3 , Jérôme Randon 1 , Olivier Tillement 2 , François Lux 2, 4 , Agnès Hagège 1
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During past decade, special focus has been laid on ultrasmall nanoparticles for nanomedicine and eventual clinical translation. To achieve such translation, a lot of challenges have to be solved. Among them, size determination is a particularly tricky one. In this aim, we have developed a simple hyphenation between Taylor dispersion analysis and inductively coupled plasma-mass spectrometry (ICP-MS). This method was proven to allow the determination of the hydrodynamic radius of metal-containing nanoparticles, even for sizes under 5 nm, with a relative standard deviation below 10% (with a 95% confidence interval) and at low concentrations. Moreover, its specificity provides the opportunity to perform measurements in complex biological media. This was applied to the characterization of an ultrasmall gadolinium-containing nanoparticle used as a theranostic agent in cancer diseases. Hydrodynamic radii measured in urine, cerebrospinal fluid, and undiluted serum demonstrated the absence of interaction between the particle and biological compounds such as proteins.
中文翻译：
泰勒色散分析与电感耦合等离子体质谱联用，用于超小纳米粒度测量：从药品到生物介质研究
在过去的十年中，一直特别关注用于纳米医学和最终临床翻译的超小纳米颗粒。为了实现这种翻译，必须解决许多挑战。其中，尺寸确定是一个特别棘手的问题。为此，我们在泰勒色散分析和电感耦合等离子体质谱法（ICP-MS）之间建立了简单的连接。实践证明，该方法即使在5 nm以下，相对标准偏差低于10％（置信区间为95％）且浓度较低的情况下，也可以测定含金属的纳米颗粒的流体力学半径。此外，其特异性为在复杂的生物介质中进行测量提供了机会。将其用于表征癌症疾病中用作治疗治疗剂的超小含g纳米颗粒。在尿液，脑脊液和未稀释的血清中测得的流体动力学半径表明，颗粒与生物化合物（例如蛋白质）之间没有相互作用。
更新日期：2021-01-26
中文翻译：
泰勒色散分析与电感耦合等离子体质谱联用，用于超小纳米粒度测量：从药品到生物介质研究
在过去的十年中，一直特别关注用于纳米医学和最终临床翻译的超小纳米颗粒。为了实现这种翻译，必须解决许多挑战。其中，尺寸确定是一个特别棘手的问题。为此，我们在泰勒色散分析和电感耦合等离子体质谱法（ICP-MS）之间建立了简单的连接。实践证明，该方法即使在5 nm以下，相对标准偏差低于10％（置信区间为95％）且浓度较低的情况下，也可以测定含金属的纳米颗粒的流体力学半径。此外，其特异性为在复杂的生物介质中进行测量提供了机会。将其用于表征癌症疾病中用作治疗治疗剂的超小含g纳米颗粒。在尿液，脑脊液和未稀释的血清中测得的流体动力学半径表明，颗粒与生物化合物（例如蛋白质）之间没有相互作用。
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