Toward Decentralizing Antibiotic Susceptibility Testing via Ready-to-Use Microwell Array and Resazurin-Aided Colorimetric Readout,Analytical Chemistry

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Toward Decentralizing Antibiotic Susceptibility Testing via Ready-to-Use Microwell Array and Resazurin-Aided Colorimetric Readout
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-12-29 , DOI: 10.1021/acs.analchem.0c04095
Fan-En Chen ₁ , Aniruddha Kaushik ₂ , Kuangwen Hsieh ₂ , Emily Chang ₁ , Liben Chen ₂ , Pengfei Zhang ₁ , Tza-Huei Wang _{1,

2,

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Affiliation

In the face of the global threat from drug-resistant superbugs, there remains an unmet need for simple and accessible diagnostic tools that can perform important antibiotic susceptibility testing against pathogenic bacteria and guide antibiotic treatments outside of centralized clinical laboratories. As a potential solution to this important problem, we report herein the development of a microwell array-based resazurin-aided colorimetric antibiotic susceptibility test (marcAST). At the core of marcAST is a ready-to-use microwell array device that is preassembled with custom titers of various antibiotics and splits bacterial samples upon a simple syringe injection step to initiate AST against all antibiotics. We also employ resazurin, which changes from blue to pink in the presence of growing bacteria, to accelerate and enable colorimetric readout in our AST. Even with its simplicity, marcAST can accurately measure the minimum inhibitory concentrations of reference bacterial strains against common antibiotics and categorize the antibiotic susceptibilities of clinically isolated bacteria. With more characterization and refinement, we envision that marcAST can become a potentially useful tool for performing AST without trained personnel, laborious procedures, or bulky instruments, thereby decentralizing this important test for combating drug-resistant superbugs.

中文翻译：

通过即用型微孔阵列和刃天青辅助比色读数实现分散的抗生素敏感性测试

面对来自耐药超级细菌的全球威胁，对简单易用的诊断工具的需求仍未得到满足，这些工具可以对病原菌进行重要的抗生素敏感性测试，并在集中临床实验室之外指导抗生素治疗。作为这一重要问题的潜在解决方案，我们在此报告了基于微孔阵列的刃天青辅助比色抗生素药敏试验 (marcAST) 的开发。marcAST 的核心是一个即用型微孔阵列设备，它预先组装了各种抗生素的定制滴度，并在一个简单的注射器注射步骤中分离细菌样本，以启动针对所有抗生素的 AST。我们还使用刃天青，在细菌生长的情况下，它会从蓝色变为粉红色，在我们的 AST 中加速和启用比色读数。即使简单，marcAST 也可以准确测量参考细菌菌株对常见抗生素的最低抑菌浓度，并对临床分离细菌的抗生素敏感性进行分类。随着更多的表征和改进，我们设想 marcAST 可以成为执行 AST 的潜在有用工具，无需经过培训的人员、费力的程序或笨重的仪器，从而分散这一对抗耐药超级细菌的重要测试。

更新日期：2021-01-26

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