The measurement of the volume of blood cells is important for clinical diagnosis and patient management. While digital holography microscopy has been used to obtain such information, previous off-axis setups usually involve a separated reference beam and are thus not very easy to implement. Here, we use the simple in-line Gabor setup without separation of a reference beam to measure the shape and volume of cells mounted on glass slides. Inherent to the in-line holograms, the reconstructed phase of the object is affected by the virtual image noise, producing errors in the cell volume measurement. We optimized our approach to use a single hologram without phase retrieval, increasing distance between cell and hologram plane to reduce the measurement error of cell volume to less than 6% in some instances. Therefore, the in-line Gabor setup can be a useful and simple tool to obtain volumetric and morphologic cellular information.

中文翻译：

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使用在线数字全息术测量细胞体积

血细胞体积的测量对于临床诊断和患者管理很重要。虽然数字全息显微镜已用于获取此类信息，但以前的离轴设置通常涉及分离的参考光束，因此不太容易实现。在这里，我们使用简单的在线 Gabor 设置而不分离参考光束来测量安装在载玻片上的细胞的形状和体积。固有的在线全息图，对象的重建相位受到虚拟图像噪声的影响，从而在细胞体积测量中产生误差。我们优化了我们的方法以使用单个全息图而无需相位检索，增加细胞和全息图平面之间的距离，以在某些情况下将细胞体积的测量误差降低到 6% 以下。所以，