A suite of blood chemistry parameters (including acid–base indicators and plasma electrolytes) was serially measured in blacktip sharks (Carcharhinus limbatus), captured via rod-and-reel, to gain a more thorough understanding of the physiological stress response to recreational capture. Sharks were caught both from the shore and from fishing vessels and experienced varying degrees of air exposure during handling. While all captured sharks exhibited a metabolic acidosis during the fight on the line (increasing lactate and decreasing pH and bicarbonate), the observed acidosis was compounded by a respiratory component (increasing pCO₂) in sharks removed from the water during handling. Vessel-caught sharks handled in the water exhibited significantly greater increases in lactate and glucose (0.73 ± 0.21 mmoll⁻¹ min⁻¹ and 0.81 ± 1.07 mg dl⁻¹min⁻¹, respectively) than sharks handled out of water (0.21 ± 0.17 mmoll⁻¹ min⁻¹ and −0.32 ± 1.05 mg dl⁻¹min⁻¹; p < 0.001 and p < 0.05, respectively). These findings provide insights into how differences in recreational capture methods and air exposure can mediate the origin and magnitude of capture-related stress and highlight the importance of considering both sampling time (time from capture to phlebotomy) and sampling location (in water vs. out) in studies conducted on capture-related stress.

中文翻译：

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深入了解沿海韧带鱼中捕获和处理相关压力的起源和大小

一系列血液化学参数（包括酸碱指示剂和血浆电解质）在黑鳍鲨（Carcharhinuslimbatus）中进行了连续测量，通过钓竿和卷轴捕获，以更深入地了解休闲捕获的生理应激反应。鲨鱼是从岸边和渔船上捕获的，在处理过程中经历了不同程度的空气暴露。虽然所有捕获的鲨鱼在战斗中都表现出代谢性酸中毒（增加乳酸和降低 pH 值和碳酸氢盐），但观察到的酸中毒由呼吸成分（增加 pCO ₂) 在处理过程中从水中取出的鲨鱼。在水中处理的船只捕获的鲨鱼显示出乳酸和葡萄糖的显着增加（分别为 0.73 ± 0.21 mmol ^-1  min ^-1和 0.81 ± 1.07 mg dl ^-1 min ^-1）比在水外处理的鲨鱼（0.21 ± 0.17） mmol ^-1 min ^-1和 -0.32 ± 1.05 mg dl ^-1 min ^-1；p  <  0.001 和p  < 0.05，分别）。这些发现提供了关于休闲捕获方法和空气暴露的差异如何调节捕获相关压力的起源和大小的见解，并强调了考虑采样时间（从捕获到采血的时间）和采样位置（水中与室外）的重要性。 ) 在对捕获相关压力进行的研究中。