Euphorbia is a large genus of flowering plants with a great diversity in metabolic pattern. Testing the cytotoxic potential of fifteen Euphorbia species revealed highest activity of E. officinarum L. against CACO2 cell line (IC₅₀ 7.2 µM) and of E. lactea Haw. against HepG2 and MCF-7 cell lines (IC₅₀ 5.2 and 5.1 µM, respectively). Additionally, metabolic profiling of the fifteen tested species, using LC-HRMS, for dereplication purposes, led to the annotation of 44 natural compounds. Among the annotated compounds, diterpenoids represent the major class. Dereplication approach and multivariate data analysis are adopted in order to annotate the compounds responsible for the detected cytotoxic activity. Results of PCA come in a great accordance with results of biological testing, which emphasized the cytotoxic properties of E. lactea Haw. A similarity correlation network showed that the two compounds with the molecular formula C₁₆H₁₈O₈ and C₂₀H₃₀O₁₀, are responsible for cytotoxic activity against MCF-7 and HepG2 cell lines. Similarly, the compound with molecular formula C₁₈H₃₅NO correlates with cytotoxic activity against CACO2.

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15种大戟属植物的细胞毒活性和代谢谱分析

大戟属是开花植物的大类，其代谢模式具有很大的多样性。测试的15个细胞毒性潜力大戟物种揭示的最高活性E.良姜L.针对CACO2细胞系（IC ₅₀ 7.2μM）和E. LACTEA唧唧。对抗HepG2和MCF-7细胞系（IC ₅₀分别为5.2和5.1 µM）。此外，出于重复的目的，使用LC-HRMS对15种受试物种进行了代谢谱分析，从而注释了44种天然化合物。在带注释的化合物中，二萜类化合物是主要的化合物。采用重复数据删除方法和多变量数据分析来注释负责检测到的细胞毒活性的化合物。PCA的结果与生物学测试的结果高度吻合，后者强调了大肠杆菌的细胞毒性。相似性相关网络显示这两种分子式为C ₁₆ H ₁₈ O ₈和C ₂₀ H ₃₀ O _10的化合物负责对MCF-7和HepG2细胞系的细胞毒活性。类似地，具有分子式C ₁₈ H ₃₅ NO的化合物与针对CACO 2的细胞毒性活性相关。