Genus Chirostoma belongs to Atherinopsidae family and it is an endemic species from the Mesa Central in Mexico. Abundance of its species have decreased and some ones have been placed on the threatened species list, because of overfishing, urbanization, industrialization, destruction, habitat fragmentation, pollution and exotic species introduction. Chirostoma jordani (Woolman, 1894) is a freshwater fish with biological, ecological, cultural, and commercial importance. It has a broad distribution in Lerma drainage, Durango and Mexico City. In this last locality, their populations, although small, still persist in Xochimilco Lake; it is necessary to implement biotechnologies for their conservation, because of these causes and their basic biology. The aim was to standardize a sperm cryopreservation protocol in C. jordani, to determine extender solution, cryoprotective agent type and concentration, equilibrium time, freezing and thawed rate to be applied in assisted reproduction and conservation of genus Chirostoma. Chirostoma jordani adult males were collected in Atlangatepec Dam, Tlaxcala State, Mexico, to fresh seminal evaluation and cryopreservation protocol standardization. Four cryoprotectants effect was evaluated: dimethylsulfoxide (DMSO), methanol (MeOH), ethylene glycol (EG), and glycerol (GL) at five concentrations: 2%, 6%, 10%, 14% and 16% v/v. Higher and lower DMSO and MeOH 10% and EG 14%, decreased post‐thaw motility percentage. Both DMSO and MeOH 10% and EG 14% had the highest post‐thaw motility percentages, 48.8 ± 1.5%, 54.5 ± 1.0% and 53.5 ± 1.0%, at 15, 10, and 5 min equilibrium times, respectively, thawed at 40°C. Chirostoma jordani sperm can be cryopreserved with both DMSO and MeOH 10%, and EG 14%. These ones can be used for assisted reproduction. GL was not efficient, since it presented a post‐thaw motility percentage very low.

中文翻译：

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墨西哥黑手鱼精子的冷冻保存，鱼类模型的保存

属Chirostoma属于Atherinopsidae家庭，是从台地中心在墨西哥特有物种。由于过度捕捞，城市化，工业化，破坏，生境破碎，污染和引入外来物种，其物种丰富度有所下降，一些物种已列入濒危物种名单。约氏假单胞菌（Woolman，1894）是一种淡水鱼，具有生物学，生态，文化和商业重要性。它在莱尔马排水，杜兰戈和墨西哥城具有广泛的分布。在这最后一个地方，他们的人口虽然很少，但仍留在霍奇米尔科湖；由于这些原因及其基本生物学，有必要实施生物技术进行保护。目的是标准化约氏假单胞菌中的精子冷冻保存方案，以确定补充剂溶液，冷冻保护剂类型和浓度，平衡时间，冷冻和解冻速率，以用于辅助手足癣属的繁殖和保存。约氏假单胞菌在墨西哥特拉克斯卡拉州Atlangatepec大坝收集成年雄性，以进行新的开创性评估和冷冻保存方案标准化。评估了四种防冻剂效果：五种浓度的二甲亚砜（DMSO），甲醇（MeOH），乙二醇（EG）和甘油（GL）：v / v分别为2％，6％，10％，14％和16％。较高和较低的DMSO和MeOH 10％和EG 14％，降低了解冻后的活力百分比。DMSO，10％的MeOH和14％的EG的融化后活力百分比最高，分别在15、10和5分钟的平衡时间解冻后为48.8±1.5％，54.5±1.0％和53.5±1.0％，在40融化时℃。约氏假单胞菌可以用10％的DMSO和MeOH，以及14％的EG冷冻保存精子。这些可以用于辅助复制。GL效率不高，因为其解冻后的运动百分比非常低。