Human leukocyte antigen B27 (HLA-B27) is an major histocompatibility complex Class I cell surface antigen that shows strong association with spondylarthropathies. Although polymerase chain reaction (PCR) is the gold standard method for HLA-B27 detection, monoclonal antibodies, and flow cytometric analysis is also frequently used. We aimed to compare the efficiency of two commercially available monoclonal antibody clones and the DuraClone kit that uses simultaneously these clones.

中文翻译：

---

不同抗体克隆联合使用提高流式细胞仪检测人白细胞抗原B27的效率

人类白细胞抗原 B27 (HLA-B27) 是一种主要的组织相容性复合体 I 类细胞表面抗原，与脊柱关节病密切相关。虽然聚合酶链式反应 (PCR) 是 HLA-B27 检测的金标准方法，但单克隆抗体和流式细胞术分析也经常使用。我们旨在比较两种市售单克隆抗体克隆和同时使用这些克隆的 DuraClone 试剂盒的效率。