Hematopoietic stem and progenitor cell (HSPC) transplantation is the paradigm for stem cell therapies. The protocol described here enables quantitative assessment of the body-wide HSPC reconstitution of different mature hematopoietic cells in mice based on their presence in circulating blood. The method determines donor-derived mature cell populations per mouse, over time, by quantitatively obtaining their absolute numbers in the peripheral blood and utilizing previously assessed tissue-distribution factors. A Markov-based birth/death computational model accounts for the drastic differences in mature cell half-lives. By quantifying the number of cells produced and eliminating host variability, the protocol can be used to directly compare the lineage output of different types of HSPCs on a per cell basis, thereby clarifying the lineage potential and expansion capacity of different cell populations. These protocols were developed for hematopoiesis, but can readily be extended to other contexts by simply replacing the cell types and distributions.

造血干细胞和祖细胞（HSPC）移植是干细胞治疗的范例。这里描述的协议能够根据小鼠循环血液中的存在对不同成熟造血细胞的全身 HSPC 重建进行定量评估。该方法通过定量获得外周血中的绝对数量并利用先前评估的组织分布因素来确定每只小鼠供体来源的成熟细胞群随时间的变化。基于马尔可夫的出生/死亡计算模型解释了成熟细胞半衰期的巨大差异。通过量化产生的细胞数量并消除宿主变异性，该协议可用于直接比较每个细胞的不同类型 HSPC 的谱系输出，从而阐明不同细胞群的谱系潜力和扩增能力。这些协议是为造血而开发的，但可以通过简单地替换细胞类型和分布来轻松扩展到其他环境。