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Silver nanoparticles synthesized by Nigrospora oryzae showed antifungal activity
Saudi Journal of Biological Sciences Pub Date : 2020-12-29 , DOI: 10.1016/j.sjbs.2020.12.036
Turki M Dawoud 1 , Mohamed A Yassin 1, 2 , Abdel Rahim M El-Samawaty 1, 2 , Abdallah M Elgorban 1, 2, 3
Affiliation  

In this investigation, an alternate green-route based on myco-synthesised silver nanoparticles (Ag NPs) was evaluated to control plant disease to reduce the usage of synthetic chemicals. Here, we described biologically synthesised Ag NPs using the corn grain contaminant, Nigrospora oryzae, and were well-characterised by UV–visible spectrophotometer, X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Energy Dispersive Spectroscopy (EDS) and particle size analyzer. The pathogenic behaviour of the Fusarium spp. were checked on Giza 86 and Giza 90 cultivars under greenhouse conditions. F. moniliforme and F. oxysporum exhibited high pathogenecity against Giza 90 and Giza 86 cultivars respectively. The antifungal activity of biosynthesised Ag NPs was evaluated against eight species of Fusaria causing damping-off of cotton seedlings. In vitro treatments with different concentrations of Ag NPs were achieved on Czapek Dox agar and Potato dextrose agar plates. Fungal growth was drastically retarded from 25 to 200 ppm of Ag NPs interaction. The antifungal activity of Ag NPs against the Fusarium spp. was clearly proven.



中文翻译:


米黑孢子菌合成的银纳米颗粒显示出抗真菌活性



在这项研究中,评估了一种基于真菌合成的银纳米颗粒(Ag NP)的替代绿色路线,以控制植物病害,从而减少合成化学品的使用。在这里,我们描述了使用玉米粒污染物米黑孢菌生物合成的银纳米颗粒,并通过紫外可见分光光度计、X射线粉末衍射(XRD)、透射电子显微镜(TEM)、能量色散光谱(EDS)进行了充分表征和粒度分析仪。镰刀菌属的致病行为。在温室条件下对 Giza 86 和 Giza 90 品种进行了检查。串珠镰刀菌尖孢镰刀菌分别对Giza 90和Giza 86品种表现出高致病性。生物合成的纳米银颗粒对引起棉苗猝倒病的八种镰孢菌的抗真菌活性进行了评估。在 Czapek Dox 琼脂和马铃薯葡萄糖琼脂平板上进行了不同浓度 Ag NP 的体外处理。 25 至 200 ppm 的银纳米颗粒相互作用会显着抑制真菌生长。 Ag NPs 对镰刀菌属的抗真菌活性。被明确证明。

更新日期:2021-03-04
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