The multi-drug resistant effect of the Gram negative bacteria K. pneumoniae was identified by disc diffusion method using specific UTI panel discs of Kleb 1 HX077 and Kleb 2 HX090 HEXA. Among the multi-drug resistant bacteria, the carbapenem resistant (CR) effect of the K. pneumoniae was screened by specific carbapenem detection antibiotics of HEXA HX066 and HX0103 HEXA by disc diffusion method. In addition, the effective antibiotics were further performed against K. pneumoniae by minimum inhibition concentration method. Further, the carbapenemase genes of VIM 1 and IMP 1 were detected from the isolated strains by multiplex PCR method. Furthermore, the biofilm forming ability of selected carbapenem resistant K. pneumoniae was initially identified by tissue culture plate method and confirmed by exopolysaccharide arrest ability of congo red agar assay. Finally, our result was proved that the identified K. pneumoniae is carbapenemase producing strain, and its virulence was extended with strong biofilm formation.

使用Kleb 1 HX077和Kleb 2 HX090 HEXA的特定UTI盘片通过盘扩散法鉴定革兰氏阴性菌肺炎克雷伯菌的多重耐药作用。在多重耐药菌中，采用纸片扩散法采用HEXA HX066和HX0103 HEXA特异性碳青霉烯类检测抗生素筛选肺炎克雷伯菌的碳青霉烯类耐药（CR）效应。此外，还采用最小抑菌浓度法进一步筛选了对肺炎克雷伯菌有效的抗生素。进一步，通过多重PCR方法从分离菌株中检测到VIM 1和IMP 1的碳青霉烯酶基因。此外，通过组织培养平板法初步鉴定了所选碳青霉烯类耐药肺炎克雷伯菌的生物膜形成能力，并通过刚果红琼脂测定的胞外多糖阻滞能力进行了证实。最后，我们的结果证明，所鉴定的肺炎克雷伯菌是产碳青霉烯酶菌株，其毒力增强，生物膜形成能力强。