Here we prepared a low-degree-sulfated κ/β-carrageenan oligosaccharide (L-DS-KOS) by DMSO-methanol desulfation method, and fabricated a sponge dressing for the wound healing of diabetic rats. The immunomodulatory effects of L-DS-KOS on M1-like macrophages were evaluated. Results showed that L-DS-KOS could effectively promote the secretion of anti-inflammatory factors and accelerate polarization of LPS-activated macrophages from M1 to M2 type. The gross examination result showed that the sponge dressing with the mass ratio of L-DS-KOS: collagen = 3: 7 could effectively accelerate the repair process of the full-thickness excisional wound in diabetic rats; and H&E and Masson staining results disclosed that the L-DS-KOS/collagen dressing could better shorten the inflammation period of the wound site, and improve the process of collagen deposition and epithelial formation, thereby promote the repair of skin wounds in diabetic rats. These results demonstrate that L-DS-KOS has potential to be used as a new type of immunomodulatory biomaterial for diabetic wound dressings.

在这里，我们通过DMSO-甲醇脱硫法制备了低度硫酸化的κ/β-角叉菜胶低聚糖（L-DS-KOS），并制备了用于糖尿病大鼠伤口愈合的海绵敷料。评估了L-DS-KOS对M1样巨噬细胞的免疫调节作用。结果表明，L-DS-KOS可以有效促进抗炎因子的分泌，并促进LPS激活的巨噬细胞从M1型转变为M2型。粗略检查结果表明，L-DS-KOS：胶原蛋白= 3：7的海绵敷料可有效促进糖尿病大鼠全层切除创面的修复。H＆E和Masson染色结果显示，L-DS-KOS /胶原蛋白敷料可以更好地缩短伤口发炎的时间，并改善胶原蛋白沉积和上皮形成的过程，从而促进糖尿病大鼠皮肤伤口的修复。这些结果表明，L-DS-KOS有潜力用作糖尿病伤口敷料的新型免疫调节生物材料。