To some extent, cell therapy for myocardial infarction (MI) has supported the idea of cardiac repair; however, further optimizations are inevitable. Combined approaches that comprise suitable cell sources and supporting molecules considerably improved its effect. Here, we devised a strategy of simultaneous transplantation of human cardiac progenitor cells (CPCs) and an optimized oxygen generating microparticles (MPs) embedded in fibrin hydrogel, which was injected into a left anterior descending artery (LAD) ligating-based rat model of acute myocardial infarction (AMI). Functional parameters of the heart, particularly left ventricular systolic function, markedly improved and reached pre-AMI levels. This functional restoration was well correlated with substantially lower fibrotic tissue formation and greater vascular density in the infarct area. Our novel approach promoted CPCs retention and differentiation into cardiovascular lineages. We propose this novel co-transplantation strategy for more efficient cell therapy of AMI which may function by providing an oxygen-rich microenvironment, and thus regulate cell survival and differentiation.

在某种程度上，用于心肌梗塞（MI）的细胞疗法支持了心脏修复的想法。但是，进一步的优化是不可避免的。包含合适细胞源和支持分子的组合方法大大改善了其效果。在这里，我们设计了一种同时移植人心脏祖细胞（CPC）和嵌入纤维蛋白水凝胶中的优化的产氧微粒（MPs）的策略，该蛋白被注射到左前降支（LAD）结扎的急性大鼠模型中心肌梗塞（AMI）。心脏的功能参数，特别是左心室收缩功能明显改善并达到AMI前水平。这种功能性恢复与梗塞区域的纤维化组织形成明显减少和血管密度更高相关。我们的新方法促进了CPC的保留并分化为心血管谱系。我们提出了这种新型的共移植策略，用于更有效的AMI细胞治疗，它可能通过提供富氧的微环境而起作用，从而调节细胞存活和分化。