Tephrosia purpurea (T. purpurea), a plant belonging to Fabaceae (pea) family, is a well-known Ayurvedic herb and commonly known as Sarapunkha in traditional Indian medicinal system. Described as “Sarwa wranvishapaka”, i.e. having a capability to heal all types of wounds, it is particularly recognized for its usage in splenomegaly. Towards exploring the comprehensive effects of T. purpurea against polycystic ovarian syndrome (PCOS) and three comorbid neuropsychiatric diseases (anxiety, depression, and bipolar disorder), its constituent phytochemicals (PCs) were extensively reviewed and their network pharmacology evaluation was carried out in this study. The complex regulatory potential of its 76 PCs against PCOS is enquired by developing and analyzing high confidence tripartite networks of protein targets of each phytochemical at both pathway and disease association scales. We also developed a high-confidence human Protein- Protein Interaction (PPI) sub-network specific to PCOS, explored its modular architecture, and probed 30 drug-like phytochemicals (DPCs) having multi-module regulatory potential. The phytochemicals showing good binding affinity towards their protein targets were also evaluated for similarity against currently available approved drugs present in DrugBank. Multi-targeting and synergistic capacities of 12 DPCs against 10 protein targets were identified and evaluated using molecular docking and interaction analyses. Eight DPCs as a potential source of PCOS and its comorbidity regulators are reported in T. purpurea. The results of network-pharmacology study highlight the therapeutic relevance of T. purpurea as PCOS-regulator and demonstrate the effectiveness of the approach in revealing action-mechanism of Ayurvedic herbs from holistic perspective.

Tephrosia purpurea（T. purpurea）是一种隶属于豆科（豌豆）的植物，是一种著名的印度草药，在印度传统医药系统中通常被称为Sarapunkha。被描述为“ Sarwa wranvishapaka ”，即具有治愈所有类型伤口的能力，尤其因其在脾肿大中的使用而闻名。探索紫锥菊的综合效应针对多囊卵巢综合征（PCOS）和三种并存的神经精神疾病（焦虑症，抑郁症和双相情感障碍），对其构成植物化学物质（PCs）进行了广泛的综述，并对其网络药理学进行了评估。通过在途径和疾病关联范围内开发和分析每种植物化学物质的蛋白质靶标的高可信度三方网络，可以查询到其76台PC对PCOS的复杂监管潜力。我们还开发了PCOS特有的高可信度人蛋白质-蛋白质相互作用（PPI）子网络，探索了其模块化体系结构，并探查了30种具有多模块调节潜力的药物样植物化学物质（DPC）。还评估了对它们的蛋白质靶标显示出良好结合亲和力的植物化学物质与目前在DrugBank中获得批准的药物的相似性。使用分子对接和相互作用分析，鉴定和评估了12个DPC对10个蛋白质目标的多靶点和协同能力。据报道，有八种DPC作为PCOS及其共病调节剂的潜在来源。紫杉 网络药理研究的结果突出了紫杉作为PCOS调节剂的治疗意义，并从整体角度证明了该方法在揭示印度草药的作用机理方面的有效性。