We describe a new biosensor platform for rapid and simple quantification of total aerobic viable counts of bacteria (TVC) in food and environmental swabs by oxygen respirometry. The system uses disposable swab vials with phosphorescent oxygen sensors integrated in the bottom part, a small block heater/incubator and a handheld sensor reader. In the testing, groups of 1–20 swabs samples were prepared using the standard method (ISO, 18593:2018) in sensor vials, which were then incubated at 30 °C and measured hourly in a contactless, non-invasive manner. The measurements reveal time profiles of dissolved O₂ in each sample vial, from which Threshold Time of sensor signal was determined and then TVC values (CFU/cm²) were calculated using the calibration equation. The method covers the range of 0.65–7.87 Log (CFU/cm²) and produces results in 1–8 hrs. The test was validated with swab samples from surfaces contaminated with E. coli, with whole meat microbiota, and with real environmental swabs. The results showed no statistically significant difference with the reference method which takes 48–72 h. The swab testing platform is fast and accurate, simple (sample-and-measure), portable, low cost (<$5k), requires no serial dilutions and is suitable for on-site deployment and use.

我们描述了一个新的生物传感器平台，用于通过氧气呼吸测定法快速简单地定量食物和环境拭子中细菌的总需氧菌活菌数（TVC）。该系统使用一次性棉签小瓶，其底部集成了磷光氧气传感器，一个小块加热器/培养箱和一个手持式传感器读取器。在测试中，使用标准方法（ISO，18593：2018）在传感器小瓶中制备1-20个拭子样品组，然后将其在30°C下温育，并以无接触，无创方式每小时测量一次。测量显示每个样品瓶中溶解的O _2的时间曲线，从中确定传感器信号的阈值时间，然后确定TVC值（CFU / cm ²）是使用校准方程计算的。该方法覆盖0.65–7.87 Log（CFU / cm ²）的范围，并在1–8小时内产生结果。该测试已用受大肠杆菌污染的表面，全肉微生物群和实际环境拭子的拭子样本进行了验证。结果显示，与参考方法相比，需要48-72小时，无统计学差异。拭子测试平台快速，准确，简单（采样和测量），便携式，低成本（<5k美元），无需连续稀释，适合现场部署和使用。