The worldwide epidemic of novel coronavirus disease (COVID-19) has led to a strong demand for highly efficient immunobinding to achieve rapid and accurate on-site detection of SARS-CoV-2 antibodies. However, hour-scale time-consumption is usually required to ensure the adequacy of immunobinding on expensive large instruments in hospitals, and the common false negative or positive results often occur in rapid on-site immunoassay (e.g. immunochromatography). We solved this dilemma by presenting a reciprocating-flowing immunobinding (RF-immunobinding) strategy. RF-immunobinding enabled the antibodies in fluid contacting with the corresponding immobilized antigens on substrate repeatedly during continuous reciprocating-flowing, to achieve adequate immunobinding within 60 s. This strategy was further developed into an immunoassay method for the serological detection of 13 suspected COVID-19 patients. We obtained a 100% true negative and true positive rate and a limit of quantification (LOQ) of 4.14 pg/mL. Our strategy also can be a potential support for other areas related to immunorecognition, such as proteomics, immunopharmacology and immunohistochemistry.

新型冠状病毒疾病（COVID-19）的全球流行导致对高效免疫结合的强烈需求，以实现对SARS-CoV-2抗体的快速，准确的现场检测。但是，通常需要几小时的时间消耗，以确保在医院昂贵的大型仪器上进行免疫结合的充分性，并且在快速的现场免疫分析中通常会出现常见的假阴性或阳性结果（例如免疫层析）。我们通过提出往复流动的免疫结合（RF-免疫结合）策略解决了这一难题。RF免疫结合使抗体能够在连续往复流动期间反复与底物上相应的固定抗原流体接触，从而在60 s内实现足够的免疫结合。将该策略进一步发展为一种免疫测定方法，用于对13名可疑COVID-19患者进行血清学检测。我们获得了100％的真实阴性和真实阳性率以及4.14 pg / mL的定量限（LOQ）。我们的策略也可以为与免疫识别相关的其他领域提供潜在支持，例如蛋白质组学，免疫药理学和免疫组化。