The orbitofrontal cortex (OFC) is involved in diverse brain functions via its extensive projections to multiple target regions. There is a growing understanding of the overall outputs of the OFC at the population level, but reports of the projection patterns of individual OFC neurons across different cortical layers remain rare. Here, by combining neuronal sparse and bright labeling with a whole-brain florescence imaging system (fMOST), we obtained an uninterrupted three-dimensional whole-brain dataset and achieved the full morphological reconstruction of 25 OFC pyramidal neurons. We compared the whole-brain projection targets of these individual OFC neurons in different cortical layers as well as in the same cortical layer. We found cortical layer-dependent projections characterized by divergent patterns for information delivery. Our study not only provides a structural basis for understanding the principles of laminar organizations in the OFC, but also provides clues for future functional and behavioral studies on OFC pyramidal neurons.

眶额皮质（OFC）通过其对多个目标区域的广泛投射参与多种大脑功能。人们对群体水平上 OFC 的总体输出的了解越来越多，但关于单个 OFC 神经元跨不同皮质层的投影模式的报道仍然很少。在这里，通过将神经元稀疏和明亮标记与全脑荧光成像系统（fMOST）相结合，我们获得了不间断的三维全脑数据集，并实现了25个OFC锥体神经元的完整形态重建。我们比较了不同皮质层以及同一皮质层中这些单个 OFC 神经元的全脑投射目标。我们发现皮质层依赖的投射以不同的信息传递模式为特征。我们的研究不仅为理解 OFC 层状组织的原理提供了结构基础，而且为未来 OFC 锥体神经元的功能和行为研究提供了线索。