The cabbage aphid Brevicoryne brassicae is a notorious agricultural pest that specializes on plants of the Brassicaceae family, which are chemically defended by glucosinolates. By sequestering glucosinolates from its host plants and producing its own activating enzyme (myrosinase), this aphid employs a self-defense system against enemies paralleling that in plants. However, we know little about the metabolic fate of individual glucosinolates during aphid sequestration and activation and about the biochemical effects of this defense on aphid enemies. Here, we probed these questions focusing on B. brassicae and a predatory lacewing, Chrysoperla carnea. We found that distinct glucosinolates were accumulated by B. brassicae at different rates, with aliphatic glucosinolates being taken up more quickly than indolic ones. B. brassicae myrosinase enzymatic activities toward different glucosinolates were strongly correlated to their rates of accumulation in vivo. Surprisingly, after simulated predation, the production of toxic isothiocyanate products (ITCs) was quantitatively outweighed by less toxic products such as nitriles and ITC-conjugates. Nevertheless, the defensive cocktails significantly impaired C. carnea development. Tissue-specific quantification of glucosinolate metabolites revealed that the lacewings employ both conjugation and mobilization to reduce the toxicity of aliphatic ITCs, but these strategies were only partially effective. These results clarify the metabolic fates of glucosinolates after sequestration by an aphid herbivore and further in a higher trophic level, as well as the consequences for predator survival and development, and might be instructive for integrative pest management approaches targeting the cabbage aphid.

甘蓝蚜虫Brevicoryne brasicae是一种臭名昭著的农业害虫，专门研究十字花科的植物，这些植物受到芥子油苷的化学保护。通过从宿主植物中隔离芥子油苷并产生其自身的活化酶（黑芥子酶），该蚜虫采用了自防御系统来对抗与植物中的敌人平行的敌人。但是，我们对单个硫代葡萄糖苷在蚜虫螯合和活化过程中的代谢命运以及这种防御作用对蚜虫敌人的生化影响知之甚少。在这里，我们探讨了这些问题，重点是芸苔芽孢杆菌和掠食性草laceChrysoperla carnea。我们发现，不同的芥子油苷是由油菜芽孢杆菌积累的。脂肪族芥子油苷的吸收速度要比吲哚类更快。芸苔芽孢杆菌黑芥子酶对不同硫代芥子油苷的酶促活性与其在体内的积累速率密切相关。令人惊讶的是，经过模拟捕食后，毒性较小的产品（例如腈和ITC结合物）在数量上超过了有毒的异硫氰酸盐产品（ITC）的产量。然而，防御性鸡尾酒严重损害了角膜衣藻的发育。芥子油苷代谢物的组织特异性定量显示，草lace利用结合和动员来降低脂族ITC的毒性，但这些策略仅部分有效。这些结果阐明了在被蚜虫食草动物隔离并进一步处于较高营养水平后，芥子油苷的代谢命运，以及对捕食者生存和发育的后果，可能对以卷心菜蚜虫为害的害虫综合治理方法具有指导意义。