The purpose of this study was to explore the effects of nicotine on the activity of Streptococcus mutans (S. mutans) in soft drinks. Regular soft drinks contain large proportions of high-fructose corn syrup (HFCS), which increases the activity of S. mutans resulting in high-caries risk compared with sugar-free soft drinks. Nicotine use exhibits a strong correlation with increased S. mutans biofilm formation. The soft drinks chosen were (Coca-Cola Classic, Diet Coke, Coca-Cola Zero Sugar, Caffeine-Free Coca-Cola, Caffeine-Free Diet Coke, Caffeine-Free Coca-Cola Zero Sugar). S. mutans was grown overnight in tryptic soy broth; nicotine was diluted in tryptic soy broth supplemented with 1.0% sucrose followed by soft drinks in dilution of 1:3. Total growth absorbance and biofilm growth were determined by spectrophotometry, absorbance measured to determine biofilm formation, and metabolic activity quantified. One-way ANOVA showed a considerable effect for HFCS and caffeine in the presence of nicotine and their interaction in all measures. Results showed sugar-free caffeinated colas demonstrated significant effect in inhibiting S. mutans biofilm formation and metabolic activity with nicotine. Nicotine-induced S. mutans increased biofilm formation and metabolic activity in the presence of HFCS and caffeine in soft drinks. In conclusion, smokers should consider sugar-free caffeinated versions to minimize the chance of developing dental caries dut to the reduction of biofilm formation.

这项研究的目的是探讨尼古丁对软饮料中变形链球菌（变形链球菌）活性的影响。普通软饮料含有高比例的高果糖玉米糖浆（HFCS），与无糖软饮料相比，它增加了变形链球菌的活性，导致高龋风险。尼古丁的使用与变形链球菌生物膜形成增加有很强的相关性。选择的软饮料是（可口可乐经典，可口可乐，可口可乐零糖，无咖啡因的可口可乐，无咖啡因的可乐，无咖啡因的可口可乐零糖）。变形链球菌在胰蛋白酶大豆肉汤中生长过夜；尼古丁先在添加了1.0％蔗糖的胰蛋白酶大豆肉汤中稀释，然后在1：3的软饮料中稀释。通过分光光度法测定总生长吸收率和生物膜生长，测量吸收率以确定生物膜形成，并对代谢活性进行定量。单向方差分析显示，在尼古丁存在下，HFCS和咖啡因及其相互作用在所有方面均具有相当大的作用。结果表明，不含糖的含咖啡因的可乐在抑制变形链球菌生物膜形成和尼古丁代谢活性方面具有显着效果。尼古丁诱导的变形链球菌在软饮料中存在HFCS和咖啡因的情况下，增加了生物膜的形成和代谢活性。总之，吸烟者应考虑使用无糖的含咖啡因的产品，以最大程度地减少由于生物膜形成减少而患龋齿的机会。