Will a Non-antibiotic Metalloid Enhance the Spread of Antibiotic Resistance Genes: The Selenate Story,Environmental Science & Technology

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Will a Non-antibiotic Metalloid Enhance the Spread of Antibiotic Resistance Genes: The Selenate Story
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-12-28 , DOI: 10.1021/acs.est.0c05698
Ling-Dong Shi _{1,

2} , Qiu-Jin Xu ₁ , Jie-Yi Liu ₁ , Zi-Xian Han ₁ , Yong-Guan Zhu _{3,

4} , He-Ping Zhao _{1,

2}

Affiliation

The rapid emergence of antibiotic resistance genes (ARGs) has become an increasingly serious threat to public health. Previous studies illustrate the antibiotic-like effect of many substances. However, whether and how commonly used or existing non-antibiotic metalloids (e.g., selenate) would enhance ARG spread remains poorly known. Here, we tracked the long-term operation of a bioreactor continuously fed with selenate for more than 1000 days. Metagenomic sequencing identified 191 different ARGs, of which the total abundance increased significantly after the amendment of selenate. Network analyses showed that ARGs resisting multiple drugs had very similar co-occurrence patterns, implying a potentially larger health risk. Host classification not only indicated multidrug-resistant species but also distinguished the mechanism of ARG enrichment for vertical transfer and horizontal gene transfer. Genome reconstruction of an ARG host suggested that selenate and its bioreduction product selenite could stimulate the overproduction of intracellular reactive oxygen species, which was confirmed by the direct measurement. Bacterial membrane permeability, type IV pilus formation, and DNA repair and recombination were also enhanced, together facilitating the horizontal acquirement of ARGs. Overall, this study for the first time highlights the ARG emergence and dissemination induced by a non-antibiotic metalloid and identifies ARG as a factor to consider in selenate bioremediation.

中文翻译：

非抗生素类金属会增强抗生素抗性基因的传播：硒的故事

抗生素抗性基因（ARGs）的迅速出现已成为对公共卫生的日益严重的威胁。先前的研究表明了许多物质的类抗生素作用。然而，是否以及如何普遍使用或现有的非抗生素类金属（例如，硒酸盐）会增强ARG扩散仍知之甚少。在这里，我们跟踪了连续喂食硒酸盐超过1000天的生物反应器的长期运行情况。元基因组测序鉴定出191种不同的ARG，在硒酸修饰后总丰度显着增加。网络分析表明，抗多种药物的ARG具有相同的共现模式，这意味着潜在的更大的健康风险。宿主分类不仅表明耐多药种，而且还区分了ARG富集的垂直转移和水平基因转移机制。ARG宿主的基因组重建表明，硒酸盐及其生物还原产物亚硒酸盐可以刺激细胞内活性氧的过量产生，这直接得到了证实。细菌膜通透性，IV型菌毛形成以及DNA修复和重组也得到了增强，共同促进了ARGs的水平获取。总的来说，这项研究首次强调了非抗生素类金属诱导的ARG的出现和传播，并确定了ARG是硒酸盐生物修复中要考虑的因素。ARG宿主的基因组重建表明，硒酸盐及其生物还原产物亚硒酸盐可以刺激细胞内活性氧的过量产生，这直接得到了证实。细菌膜通透性，IV型菌毛形成以及DNA修复和重组也得到了增强，共同促进了ARGs的水平获取。总的来说，这项研究首次强调了非抗生素类金属诱导的ARG的出现和传播，并确定了ARG是硒酸盐生物修复中要考虑的因素。ARG宿主的基因组重建表明，硒酸盐及其生物还原产物亚硒酸盐可以刺激细胞内活性氧的过量产生，这直接得到了证实。细菌膜通透性，IV型菌毛形成以及DNA修复和重组也得到了增强，共同促进了ARGs的水平获取。总的来说，这项研究首次强调了非抗生素类金属诱导的ARG的出现和传播，并确定了ARG是硒酸盐生物修复中要考虑的因素。DNA的修复和重组也得到了增强，共同促进了ARGs的水平获取。总的来说，这项研究首次强调了非抗生素类金属诱导的ARG的出现和传播，并确定了ARG是硒酸盐生物修复中要考虑的因素。DNA的修复和重组也得到了增强，共同促进了ARGs的水平获取。总的来说，这项研究首次强调了非抗生素类金属诱导的ARG的出现和传播，并确定了ARG是硒酸盐生物修复中要考虑的因素。

更新日期：2021-01-19

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