Background. Early diagnosis of ankylosing spondylitis (AS) is yet not elucidated, with a potential diagnostic glance of microRNAs (miRNAs). Aim. Study the expression profile of miRNA-451a and miRNA-125a in AS patients and their impact on disease activity and prognosis. Methods. A cross-sectional study included 55 AS patients diagnosed according to modified New York criteria in 1984 with 55 matched healthy controls. History, clinical examination, and disease activity assessment with Bath ankylosing spondylitis disease activity index (BASDAI) were done. Full laboratory and radiological assessment along with expression profile of m iRNA-451a and miRNA-125a were tabulated and analyzed. Results. Higher expression levels of miRNA-125a and lower of miRNA-451a in AS patients compared to controls. Furthermore, miRNA-125a expression was high in active AS patients compared to inactive patients and controls ( vs. vs. , , respectively). miRNA-451a was significantly lower in active AS patients compared to inactive patients and controls ( vs. vs. , respectively). Both miRNAs (miRNA-125a and miRNA-451a) had evident accuracy for AS diagnosis with areas under the curve (AUC) of 0.788 and 0.802, respectively. miRNA-125a had potential impact on AS activity with AUC of 0.777. Plasma levels of both miRNAs were able to distinguish AS patients with structural damage with AUCs 0.775 and 0.692, respectively. Conclusions. Both miRNA-451a and miRNA-125a were found to be of great value as sensitive noninvasive diagnostic, prognostic, and disease burden biomarker of AS patients in Egypt with suggested further studies for future therapeutic implications.

中文翻译：

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强直性脊柱炎中的 miRNA-451a 和 miRNA-125a 表达水平：对疾病诊断、预后和结果的影响

背景。强直性脊柱炎 (AS) 的早期诊断尚未阐明，microRNA (miRNA) 具有潜在的诊断意义。瞄准。研究 miRNA - 451a 和 miRNA-125a 在 AS 患者中的表达谱及其对疾病活动和预后的影响。方法。一项横断面研究包括 1984 年根据修改后的纽约标准诊断的 55 名 AS 患者和 55 名匹配的健康对照。病史、临床检查和疾病活动性评估采用巴斯强直性脊柱炎疾病活动指数（BASDAI）。将完整的实验室和放射学评估以及 m iRNA-451a 和 miRNA-125a 的表达谱制成表格并进行分析。结果. 与对照组相比，AS 患者中 miRNA-125a 的表达水平较高，而 miRNA-451a 的表达水平较低。此外，与不活动的患者和对照组相比，活动性 AS 患者的 miRNA-125a 表达较高。对比对比, ，分别）。与非活动性患者和对照组相比，活动性 AS 患者的 miRNA-451a 显着降低。对比对比，分别）。两种 miRNA（miRNA-125a 和 miRNA-451a）对 AS 诊断具有明显的准确性，曲线下面积（AUC）分别为 0.788 和 0.802。miRNA-125a 对 AS 活性有潜在影响，AUC 为 0.777。两种 miRNA 的血浆水平能够区分具有结构损伤的 AS 患者，AUC 分别为 0.775 和 0.692。结论。发现 miRNA-451a 和 miRNA-125a 作为敏感的非侵入性诊断、预后和埃及 AS 患者疾病负担的生物标志物具有重要价值，并为未来的治疗意义提出了进一步的研究建议。