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Integrated SERS Platform for Reliable Detection and Photothermal Elimination of Bacteria in Whole Blood Samples
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-12-28 , DOI: 10.1021/acs.analchem.0c03981 Xia Gao 1 , Yanliang Yin 1 , Haotian Wu 1 , Zhe Hao 1 , Jinjie Li 1 , Shuo Wang 2 , Yaqing Liu 1, 3
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-12-28 , DOI: 10.1021/acs.analchem.0c03981 Xia Gao 1 , Yanliang Yin 1 , Haotian Wu 1 , Zhe Hao 1 , Jinjie Li 1 , Shuo Wang 2 , Yaqing Liu 1, 3
Affiliation
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Herein, an interference-free surface-enhanced Raman scattering (SERS) platform with a “sandwich” structure has been developed for reliable detection and photothermal killing of bacteria with whole blood as the real sample. The multifunctional platform comprised a plasmonic gold film (pAu) functionalized with bacteria-capturing units of 4-mercaptophenylboronic acid and internal reference of 4-mercaptobenzonitrile as the SERS substrate and vancomycin-modified core (gold)–shell (Prussian blue) nanoparticles (Au@PB@Van NPs) as the SERS tag. The detected SERS signals were from the Raman-silent region where no background signals occurred from biological sources, eliminating the interference and improving the detection sensitivity and accuracy. As a proof-of-concept, model bacterial strain, Staphylococcus aureus, was captured and detected in the whole blood samples. Furthermore, high antibacterial efficiency of approximately 100% was reached under the synergistic photothermal effect from pAu and Au@PB@Van NPs. This study provides a new avenue for bacteria detection in real samples and their subsequent in situ elimination.
中文翻译：
集成的SERS平台，可对全血样品中的细菌进行可靠的检测和光热消除
在此，已开发出具有“三明治”结构的无干扰表面增强拉曼散射（SERS）平台，用于可靠检测和光热杀死以全血为真实样本的细菌。多功能平台包括一个等离子金膜（pAu），该膜用4-巯基苯基硼酸的细菌捕获单元和4-巯基苄腈的内部参照物作为SERS底物和万古霉素修饰的核（金）-壳（普鲁士蓝）纳米颗粒（Au） @ PB @ Van NPs）作为SERS标签。检测到的SERS信号来自拉曼沉默区，那里没有生物来源的背景信号，从而消除了干扰并提高了检测灵敏度和准确性。作为概念验证的模型细菌菌株金黄色葡萄球菌被捕获并在全血样本中检测到。此外，在来自pAu和Au @ PB @ Van NP的协同光热作用下，达到了约100％的高抗菌效率。该研究为实际样品中的细菌检测以及随后的原位消除提供了新途径。
更新日期：2021-01-26
中文翻译：
集成的SERS平台，可对全血样品中的细菌进行可靠的检测和光热消除
在此，已开发出具有“三明治”结构的无干扰表面增强拉曼散射（SERS）平台，用于可靠检测和光热杀死以全血为真实样本的细菌。多功能平台包括一个等离子金膜（pAu），该膜用4-巯基苯基硼酸的细菌捕获单元和4-巯基苄腈的内部参照物作为SERS底物和万古霉素修饰的核（金）-壳（普鲁士蓝）纳米颗粒（Au） @ PB @ Van NPs）作为SERS标签。检测到的SERS信号来自拉曼沉默区，那里没有生物来源的背景信号，从而消除了干扰并提高了检测灵敏度和准确性。作为概念验证的模型细菌菌株金黄色葡萄球菌被捕获并在全血样本中检测到。此外，在来自pAu和Au @ PB @ Van NP的协同光热作用下，达到了约100％的高抗菌效率。该研究为实际样品中的细菌检测以及随后的原位消除提供了新途径。
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