Submergence and waterlogging lead to significant reductions in crop productivity and trigger dramatic changes in gene expression of plant biotic/abiotic stress response. Several of the host factors are involved in low-oxygen stress that is induced by endogenous reactive oxygen species (ROS) accumulation. Hypoxia-response unknown protein (HUP) has been found as a host factor of hypoxia screening but HUPs function largely is unknown. In this study, we found the Arabidopsis HUP26 gene which was conserved in different plant species and responded to various oxidative stress. HUP26 promoter analysis showed GUS activity in root and leaf tissues was significantly responsive to oxidative stress. HUP26-GFP is predominantly located in the cytoplasmic region. HUP26 overexpression results in altered enhanced pathogenesis-related gene 1 gene expression and reduced ion leakage levels compared with hup26 knockout and WT plants after inoculation with Pst DC3000. HUP26 overexpression transgenic plants showed improved resistance to Pst DC3000, but hup26 knockout plants exhibited increased susceptibility. Collectively, these results indicate that HUP26 plays important role in responses to various oxidative stress and confers biotic stress resistance. Engineering of HUP26 gene expression may represent a strategy to enhance biotic stress resistance of crops.

中文翻译：

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缺氧响应未知蛋白在增强生物胁迫抗性中的新功能

淹没和涝渍导致作物生产力显着降低，并引发植物生物/非生物胁迫响应基因表达的巨大变化。一些宿主因素涉及由内源性活性氧 (ROS) 积累诱导的低氧应激。缺氧反应未知蛋白 (HUP) 已被发现作为缺氧筛查的宿主因素，但 HUP 的功能在很大程度上是未知的。在本研究中，我们发现了拟南芥HUP26基因，该基因在不同植物物种中保守并响应各种氧化应激。HUP26 启动子分析表明根和叶组织中的 GUS 活性对氧化应激有显着响应。HUP26-GFP 主要位于细胞质区域。与接种 Pst DC3000 后的 hup26 敲除和 WT 植物相比，HUP26 过表达导致发病机制相关基因 1 基因表达增强，离子泄漏水平降低。HUP26 过表达转基因植物对 Pst DC3000 的抗性提高，但 hup26 敲除植物表现出增加的易感性。总的来说，这些结果表明 HUP26 在对各种氧化应激的反应中起重要作用，并赋予生物胁迫抗性。HUP26 基因表达的工程化可能代表一种增强作物生物胁迫抗性的策略。这些结果表明 HUP26 在对各种氧化应激的反应中起重要作用，并赋予生物胁迫抗性。HUP26 基因表达的工程化可能代表一种增强作物生物胁迫抗性的策略。这些结果表明 HUP26 在对各种氧化应激的反应中起重要作用，并赋予生物胁迫抗性。HUP26 基因表达的工程化可能代表一种增强作物生物胁迫抗性的策略。