ABSTRACT

HBV pol plays a critical role in the replication of hepatitis B virus (HBV). Previous studies conducted on HBV pol have produced limited evidence on HBV pol expression due to the lack of effective detection methods. The present study used the HBV pol (159–406 aa) protein as a target to screen for specific monoclonal antibodies that recognize HBV pol and subsequently evaluate their diagnostic and therapeutic value. Four antibodies (P3, P5, P12, P20) against HBV pol were obtained. Among them, the P20 antibody indicated optimal binding with HBV pol as demonstrated by Western blotting (WB) in a cell model transfected with the HBV genome. We also expressed P5 and P12 antibodies in mouse liver cells by transfection and the results indicated significant antiviral effects caused by these two antibodies especially P12. In summary, the present study established an antibody which was denoted P20. This antibody can be used to detect HBV pol expression by four HBV genomes via WB analysis. In addition, the antibody denoted P12 could exert antiviral effects via intracellular expression, which may provide a promising approach for the treatment of chronic hepatitis B.

摘要

HBV pol 在乙型肝炎病毒 (HBV) 的复制中起关键作用。由于缺乏有效的检测方法，以前对 HBV pol 进行的研究对 HBV pol 表达的证据有限。本研究使用 HBV pol (159–406 aa) 蛋白作为靶标来筛选识别 HBV pol 的特定单克隆抗体，并随后评估其诊断和治疗价值。获得了四种针对 HBV pol 的抗体（P3、P5、P12、P20）。其中，P20 抗体表明与 HBV pol 的最佳结合，如蛋白质印迹 (WB) 在用 HBV 基因组转染的细胞模型中所证明的。我们还通过转染在小鼠肝细胞中表达了 P5 和 P12 抗体，结果表明这两种抗体尤其是 P12 引起了显着的抗病毒作用。总之，本研究建立了一种标记为 P20 的抗体。该抗体可用于通过 WB 分析检测四个 HBV 基因组的 HBV pol 表达。此外，表示为P12的抗体可以通过细胞内表达发挥抗病毒作用，这可能为慢性乙型肝炎的治疗提供一种有前景的方法。