Abstract

Plants of the genus Hypericum contain various types of secondary metabolites that exhibited extensive biological activities. In the ongoing efforts to discover natural neuroinflammatory inhibitors with the potential to develop into therapeutic agents for neurodegenerative diseases, two new benzophenone glycosides, hyperewalones A and B (1 and 2), along with eight known compounds (3–10), were isolated from the aerial parts of Hypericum przewalskii. Their structures were elucidated by comprehensive analysis of IR, HRESIMS, 1D and 2D NMR spectra, and chemical derivatization. The anti-neuroinflammatory activity of compounds 1–10 was evaluated by determining their ability to inhibit the production of nitric oxide (NO) in lipopolysaccharide (LPS)-activated BV-2 microglial cells. Compounds 2, 4, 6–8 exhibited significant anti-neuroinflammatory activity with IC₅₀ values of 0.61–4.90 μM. These findings suggest that the benzophenone, ionone, and flavonoid glycosides isolated from H. przewalskii are promising anti-neuroinflammatory compounds worthy of further investigations.

摘要

金丝桃属植物含有各种类型的次生代谢物，这些代谢物表现出广泛的生物活性。在发现具有发展为神经退行性疾病治疗剂潜力的天然神经炎症抑制剂的持续努力中，从金丝桃的地上部分。通过对 IR、HRESIMS、1D 和 2D NMR 光谱以及化学衍生化的综合分析，阐明了它们的结构。化合物1-10的抗神经炎症活性通过确定它们抑制脂多糖 (LPS) 激活的 BV-2 小胶质细胞中一氧化氮 (NO) 产生的能力来评估。化合物2、4、6-8表现出显着的抗神经炎症活性，IC 50_值为0.61-4.90 μM 。这些发现表明，从普氏原虫中分离出的二苯甲酮、紫罗兰酮和类黄酮苷是有前景的抗神经炎症化合物，值得进一步研究。