Abstract

This study investigated the chemical constituents of Gaultheria procumbens essential oil and is the first to relate cytogenotoxicity with oxidative metabolism and antimicrobial activity. Chromatographic analysis of the essential oil showed methyl salicylate (99.96%) and linalool (0.04%) as the major compounds. The essential oil showed no signs of cytogenotoxicity at different concentrations (1.82 to 58.34 mg mL⁻¹). Furthermore, G. procumbens essential oil and methyl salicylate were used to evaluate the minimal inhibitory concentrations (MIC) and minimal microbicidal concentrations (MMC). The results showed efficacy against several microorganisms, including Aeromonas caviae, Candida albicans, and Mycobacterium fortuitum with MIC values ranging from 1.82 to 3.64 mg mL⁻¹ and MMC values ranging from 3.64 to 12.67 mg mL⁻¹, which were confirmed by time-kill kinetics. Based on our results, the essential oil is a promising alternative to developing future formulations to treat infections caused by microorganisms.

摘要

本研究调查了Gaultheria procumbens精油的化学成分，并首次将细胞基因毒性与氧化代谢和抗菌活性联系起来。精油的色谱分析显示水杨酸甲酯 (99.96%) 和芳樟醇 (0.04%) 是主要化合物。精油在不同浓度（1.82 至 58.34 mg mL ^-1）下未显示细胞遗传毒性迹象。此外，使用G. procumbens精油和水杨酸甲酯评估最低抑菌浓度 (MIC) 和最低杀菌浓度 (MMC)。结果显示对多种微生物有效，包括Aeromonas caviae、Candida albicans和MIC 值为 1.82 至 3.64 mg mL ^-1和 MMC 值范围为 3.64 至 12.67 mg mL ^-1的偶然分枝杆菌，这通过时间杀伤动力学得到证实。根据我们的研究结果，精油是一种很有前途的替代品，可以替代开发未来治疗由微生物引起的感染的配方。