LncRNA PART1 promotes cell proliferation and progression in non‐small‐cell lung cancer cells via sponging miR‐17‐5p,Journal of Cellular Biochemistry

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LncRNA PART1 promotes cell proliferation and progression in non‐small‐cell lung cancer cells via sponging miR‐17‐5p
Journal of Cellular Biochemistry ( IF 3.0 ) Pub Date : 2020-12-27 , DOI: 10.1002/jcb.29714
Yeye Chen _{1,

2} , Xiaoyun Zhou _{1,

2} , Cheng Huang _{1,

2} , Li Li _{1,

2} , Yingzhi Qin _{1,

2} , Zhenhuan Tian _{1,

2} , Jia He _{1,

2} , Hongsheng Liu _{1,

2}

Affiliation

It has been demonstrated in previous studies that lncPART1 is dysregulated in non‐small cell lung cancer (NSCLC). However, the function of lncPART1 in NSCLC is unclear. Therefore, this experimental design was based on LncPART1 to explore the pathogenesis of NSCLC. Real‐time polymerase chain reaction was used to detect the expression of lncPART1 and miR‐17‐5p in NSCLC. Cell Counting Kit ‐8, colony formation, and transwell assays were used to examine the effects of lncPART1 and miR‐17‐5p on NSCLC cell proliferation and migration invasiveness. Target gene prediction, luciferase reporter assays were used to validate downstream target genes for lncPART1 and miR‐17‐5p. Western blot analysis was used to detect the expression of TGFBETAR2. LncPART1 was highly expressed in NSCLC. LncPART1 significantly promoted cell proliferation of NSCLC cells. miR‐17‐5p was down‐expressed in NSCLC. miR‐17‐5p overexpression inhibited cell proliferation and migration invasion in NSCLC cells. LncPART1 was able to inhibit miR‐17‐5p expression and upregulate the expression level of TGFBETAR2. The results of in vivo animal models confirmed that lncPART1 promoted NSCLC progression by miR‐17‐5p/TGFBETAR2 axis. LncPART1 promoted the progression of NSCLC by miR‐17‐5p/TGFBETAR2 axis.

中文翻译：

LncRNA PART1 通过海绵 miR-17-5p 促进非小细胞肺癌细胞增殖和进展

先前的研究已证明lncPART1在非小细胞肺癌（NSCLC）中失调。然而，lncPART1在NSCLC中的功能尚不清楚。因此，本实验设计基于LncPART1来探讨NSCLC的发病机制。采用实时聚合酶链反应检测NSCLC中lncPART1和miR-17-5p的表达。使用细胞计数试剂盒‐8、集落形成和 Transwell 实验检测 lncPART1 和 miR-17-5p 对 NSCLC 细胞增殖和迁移侵袭的影响。靶基因预测、荧光素酶报告基因检测用于验证 lncPART1 和 miR-17-5p 的下游靶基因。 Western blot分析检测TGFBETAR2的表达。 LncPART1 在 NSCLC 中高表达。 LncPART1显着促进NSCLC细胞的增殖。 miR-17-5p 在 NSCLC 中表达下调。 miR-17-5p 过表达抑制 NSCLC 细胞的增殖和迁移侵袭。 LncPART1 能够抑制 miR-17-5p 表达并上调 TGFBETAR2 的表达水平。体内动物模型结果证实lncPART1通过miR-17-5p/TGFBETAR2轴促进NSCLC进展。 LncPART1通过miR-17-5p/TGFBETAR2轴促进NSCLC的进展。

更新日期：2021-02-16

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