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Tiron alleviates MPTP‐induced Parkinsonism in mice via activation of Keap‐1/Nrf2 pathway
Journal of Biochemical and Molecular Toxicology ( IF 3.2 ) Pub Date : 2020-12-28 , DOI: 10.1002/jbt.22685 Shrook A Mohamed 1 , Dalia H El-Kashef 1 , Manar A Nader 1
Journal of Biochemical and Molecular Toxicology ( IF 3.2 ) Pub Date : 2020-12-28 , DOI: 10.1002/jbt.22685 Shrook A Mohamed 1 , Dalia H El-Kashef 1 , Manar A Nader 1
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Parkinsonism is a neurodegenerative disease that is common all over the world. This study aimed at exploring the neuroprotective effect of tiron against 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐induced Parkinsonism. MPTP (30 mg/kg, intraperitoneally [ip]) was injected in mice daily for 5 consecutive days. Mice were treated with tiron (140 and 280 mg/kg, ip) or levodopa (8.4 mg/kg, orally) for 10 consecutive days starting 5 days before MPTP injection. At the end of the experiment, behavioral tests were conducted to assess the neuroprotective effect of tiron. Moreover, oxidative stress was assessed via measuring antioxidant enzyme, such as catalase, and lipid peroxidation was evaluated as malondialdehyde. Neuronal damage was also detected by histopathological examination and via estimating hippocampal levels of dopamine, γ‐aminobutyric acid, and nuclear factor erythroid‐derived 2‐like 2. In addition, the expression of Kelch‐like ECH‐associated protein 1 and heme oxygenase‐1 was assessed by immunohistochemistry. Compared with the blank control group and the positive control group, the inhibitory effect of tiron on MPTP‐induced neurodegenerative injury was statistically significant.
中文翻译：
Tiron通过激活Keap-1 / Nrf2途径减轻了MPTP诱发的小鼠帕金森病
帕金森病是一种神经退行性疾病，在世界各地都很普遍。这项研究旨在探讨铁蛋白对1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱发的帕金森病的神经保护作用。每天连续5天每天在小鼠中注射MPTP（30 mg / kg，腹膜内[ip]）。从MPTP注射前5天开始，连续10天用铁（140和280 mg / kg，腹腔注射）或左旋多巴（8.4 mg / kg，口服）治疗小鼠。在实验结束时，进行了行为测试以评估铁的神经保护作用。此外，通过测量抗氧化酶如过氧化氢酶来评估氧化应激，并且脂质过氧化被评估为丙二醛。还可以通过组织病理学检查和估算海马多巴胺水平来检测神经元损伤，γ-氨基丁酸和核因子类胡萝卜素衍生的2类2。此外，还通过免疫组织化学评估了Kelch类ECH相关蛋白1和血红素加氧酶1的表达。与空白对照组和阳性对照组相比，tiron对MPTP诱导的神经退行性损伤的抑制作用具有统计学意义。
更新日期：2020-12-28
中文翻译：
Tiron通过激活Keap-1 / Nrf2途径减轻了MPTP诱发的小鼠帕金森病
帕金森病是一种神经退行性疾病，在世界各地都很普遍。这项研究旨在探讨铁蛋白对1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱发的帕金森病的神经保护作用。每天连续5天每天在小鼠中注射MPTP（30 mg / kg，腹膜内[ip]）。从MPTP注射前5天开始，连续10天用铁（140和280 mg / kg，腹腔注射）或左旋多巴（8.4 mg / kg，口服）治疗小鼠。在实验结束时，进行了行为测试以评估铁的神经保护作用。此外，通过测量抗氧化酶如过氧化氢酶来评估氧化应激，并且脂质过氧化被评估为丙二醛。还可以通过组织病理学检查和估算海马多巴胺水平来检测神经元损伤，γ-氨基丁酸和核因子类胡萝卜素衍生的2类2。此外，还通过免疫组织化学评估了Kelch类ECH相关蛋白1和血红素加氧酶1的表达。与空白对照组和阳性对照组相比，tiron对MPTP诱导的神经退行性损伤的抑制作用具有统计学意义。
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