In the present work, key parameters in copper bioleaching from chalcopyrite have been investigated at long‐term operation. In detail, the type of mixed microbial consortium (origin and adaptation); the composition of two mineral media (the growth medium and the modified 9K medium); its buffer capacity by the buffers HCl/KCl and Na₂HPO₄/KH₂PO_4; and the influence of different ore grades in relation with the potential alkalinity associated have been investigated. For the first time, a mixed microbial consortium, obtained from a gas‐phase biotrickling filter treating high loads of H₂S, was employed revealing significant copper extraction by biological leaching. Results reveal that a single adaptation step of this biomass improved both kinetics and process efficiency, nearly doubling the amount of copper obtained compared with the non‐adapted consortium. Nevertheless, the growth medium also influences the efficiency of the bioleaching process, enhancing copper extraction at higher sulfate concentration. The ore containing the metal is also a determining factor, obtaining same copper extraction for biotic and abiotic in one case, and enhancing up to 50 times from the abiotic in the other. Thus, this becomes a relevant limitation for the applicability of bioleaching for some ores, mainly due to the composition of the matrix.

中文翻译：

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矿石品位和矿物介质对混合菌群黄铜矿生物浸出的影响

在目前的工作中，长期运行中已经研究了从黄铜矿生物浸出铜的关键参数。详细地讲，混合微生物联盟的类型（起源和适应）；两种矿物培养基（生长培养基和改良的9K培养基）的组成；其缓冲能力由HCl / KCl和Na ₂ HPO ₄ / KH ₂ PO ₄缓冲剂组成_。并研究了不同矿石等级与潜在碱度相关的影响。第一次是从气相生物滴滤池中获得的混合微生物联合体，用于处理高负荷的H ₂S，被用来揭示通过生物浸出显着的铜提取。结果表明，这种生物质的单个适应步骤改善了动力学和工艺效率，与未适应的财团相比，获得的铜量几乎翻了一番。然而，生长培养基也影响生物浸出过程的效率，在较高的硫酸盐浓度下促进铜的提取。含金属的矿石也是决定因素，一种情况下获得相同的铜用于生物和非生物提取，而另一种情况下则比非生物提高多达50倍。因此，这主要由于基质的组成而成为对某些矿石进行生物浸出的适用性的相关限制。