当前位置：
X-MOL 学术
›
Plant Biol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
24‐epibrassinolide‐induced growth promotion of wheat seedlings is associated with changes in the proteome and tyrosine phosphoproteome
Plant Biology ( IF 4.2 ) Pub Date : 2020-12-28 , DOI: 10.1111/plb.13233 Azamat Avalbaev 1 , Ruslan Yuldashev 1 , Kristina Fedorova 1 , Natalia Petrova 2 , Evgenia Fedina 2 , Regina Gilmanova 2 , Fatima Karimova 2 , Farida Shakirova 1
中文翻译：
24-表油菜素内酯诱导的小麦幼苗生长促进与蛋白质组和酪氨酸磷酸化蛋白质组的变化有关
更新日期：2020-12-28
Plant Biology ( IF 4.2 ) Pub Date : 2020-12-28 , DOI: 10.1111/plb.13233 Azamat Avalbaev 1 , Ruslan Yuldashev 1 , Kristina Fedorova 1 , Natalia Petrova 2 , Evgenia Fedina 2 , Regina Gilmanova 2 , Fatima Karimova 2 , Farida Shakirova 1
Affiliation
|
- Brassinosteroids (BRs) represent a unique class of steroidal plant hormones that display pronounced growth‐promoting activity at very low concentrations. Although many efforts have been made to characterize the molecular basis of BR action, little is known about the mechanisms behind the growth‐promoting effect of BRs at protein level.
- Proteomic analysis of response to the steroid plant hormone 24‐epibrassinolide (EBR) in wheat seedling shoots (Triticum aestivum L.) was performed using two‐dimensional electrophoresis (2‐DE) and immunoblotting with highly specific antibodies (PY20) to phosphotyrosine. EBR‐modulated proteins and phosphotyrosine polypeptides were identified using MALDI‐TOF mass spectrometry.
- The study revealed that EBR‐stimulated growth of wheat seedlings was accompanied by changes in the content of multiple proteins as well as in tyrosine phosphorylation of numerous polypeptides. Among them, 22 differentially accumulated proteins and 13 phosphotyrosine proteins were identified. Based on their performed functions, the identified proteins are involved in physiological processes (photosynthesis, growth, energy and amino acid metabolism) closely associated with intensification of plant metabolism.
- The EBR‐induced changes in protein abundance and tyrosine phosphorylation profile may contribute to growth stimulation of wheat seedlings under the action of EBR. The obtained data suggest an important role for EBR in the activation of protein metabolism underlying fundamental physiological processes, including growth promotion.
中文翻译：
24-表油菜素内酯诱导的小麦幼苗生长促进与蛋白质组和酪氨酸磷酸化蛋白质组的变化有关
- 油菜素类固醇（BRs）是一类独特的类固醇植物激素，在极低的浓度下显示出明显的促生长活性。尽管已做出许多努力来表征BR作用的分子基础，但对BRs在蛋白质水平上的促生长作用背后的机制知之甚少。
- 使用二维电泳（2-DE）并使用针对磷酸酪氨酸的高度特异性抗体（PY20）进行免疫印迹，对小麦幼苗新芽（Triticum aestivum L.）中对甾体植物激素24-表油菜素内酯（EBR）的响应进行蛋白质组学分析。使用MALDI-TOF质谱法鉴定了EBR调节的蛋白和磷酸酪氨酸多肽。
- 研究表明，EBR刺激的小麦幼苗生长伴随着多种蛋白质含量的变化以及许多多肽的酪氨酸磷酸化。其中，鉴定出22种差异积累的蛋白和13种磷酸酪氨酸蛋白。根据其执行的功能，鉴定出的蛋白质参与与植物代谢强度增强密切相关的生理过程（光合作用，生长，能量和氨基酸代谢）。
- EBR诱导的蛋白质丰度和酪氨酸磷酸化谱的变化可能在EBR的作用下促进小麦幼苗的生长。获得的数据表明EBR在激活包括生长促进在内的基本生理过程的蛋白质代谢中起着重要作用。
京公网安备 11010802027423号