In the present study, the protective effects of chrysin (CHR) against testicular damage caused by lead acetate (PbAc) were examined. In this way, 30 min after rats were given 25 and 50 mg/kg/b.w CHR orally for seven consecutive days, 30 mg/kg/b.w PbAc was administered orally. In biochemical analysis of testicular tissue, it was found that PbAc‐reduced antioxidant parameters [glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT)], while it increased lipid peroxidation, inflammatory markers [nuclear factor kappa‐B (NF‐κB), cyclooxygenase‐2 (COX‐2), prostaglandin E2 (PGE‐2), and inducible nitric oxide synthase (iNOS)], and 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG). In the immunohistochemical examination, it was determined that PbAc increased the expression of tumor necrosis factor‐α (TNF‐α) and caspase‐3. Accordingly, PbAc was found to cause a decrease in sperm motility and an increase in the percentage of dead sperm. However, it has been observed that CHR relieves oxidative stress due to its antioxidant properties, thus protecting against inflammation and apoptosis. It also allowed the CHR sperm parameters to return to control group levels. The results revealed that CHR could be a natural substance to be used in Pb‐induced testicular toxicity.

中文翻译：

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Chrysin具有抗氧化，抗炎和抗凋亡特性，可防止醋酸铅对大鼠的睾丸毒性

在本研究中，研究了菊花链（CHR）对乙酸铅（PbAc）引起的睾丸损伤的保护作用。这样，连续7天给大鼠口服25和50 mg / kg / bw CHR后30分钟，口服30 mg / kg / bw PbAc。在睾丸组织的生化分析中，发现PbAc降低了抗氧化剂参数[谷胱甘肽过氧化物酶（GPx），谷胱甘肽（GSH），超氧化物歧化酶（SOD）和过氧化氢酶（CAT）]，同时增加了脂质过氧化作用，炎症标志物[核因子κB（NF-κB），环加氧酶-2（COX-2），前列腺素E2（PGE-2）和诱导型一氧化氮合酶（iNOS）]和8-羟基-2'-脱氧鸟苷（8- OHdG）。在免疫组织化学检查中，确定PbAc可增加肿瘤坏死因子α（TNF-α）和caspase-3的表达。因此，发现PbAc引起精子运动性降低和精子死亡百分率增加。然而，已经观察到CHR由于其抗氧化特性而减轻了氧化应激，从而防止了炎症和细胞凋亡。它还允许CHR精子参数返回对照组水平。结果表明，CHR可能是用于铅诱导的睾丸毒性的天然物质。